碳酸酐酶Ⅲ(CA3)活性蛋白
Active Carbonic Anhydrase III, Muscle Specific (CA3)
CA-III; CAIII; Car3; Carbonate dehydratase III
- 编号APD070Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点7.4
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 暂无报价 暂无报价 暂无报价 暂无报价 暂无报价
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 H2O = HCO3- H , which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA3 is a cytosolic enzyme with a very low CA activity. It is expressed at low levels in human muscle during early development but increases rapidly during the last trimester to reach 50-60% of adult levels at birth. The activity of recombinant human CA3 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 μL of various concentrations of CA3 (diluted by assay buffer) to 50 µL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37℃ for 5min, then read at a wavelength of 400 nm.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPD070Hu01 | 碳酸酐酶Ⅲ(CA3)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APD070Hu01 | 碳酸酐酶Ⅲ(CA3)活性蛋白 | Cell culture; Activity Assays. |
| PAD070Hu01 | 碳酸酐酶Ⅲ(CA3)多克隆抗体 | WB |
| LAD070Hu71 | 碳酸酐酶Ⅲ(CA3)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAD070Hu81 | 碳酸酐酶Ⅲ(CA3)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAD070Hu22 | 碳酸酐酶Ⅲ(CA3)单克隆抗体 | WB; IHC; ICC; IP. |
| MAD070Hu23 | 碳酸酐酶Ⅲ(CA3)单克隆抗体 | WB; IHC; ICC; IP. |
| MAD070Hu21 | 碳酸酐酶Ⅲ(CA3)单克隆抗体 | WB |
| LAD070Hu72 | 碳酸酐酶Ⅲ(CA3)单克隆抗体(生物素标记) | WB; IHC; ICC. |
| SED070Hu | 碳酸酐酶Ⅲ(CA3)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD070Hu | 碳酸酐酶Ⅲ(CA3)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Biomarkers in Medicine | Biochemical markers of muscle damage and high serum concentration of creatine kinase in patients on statin therapy [Pubmed: 31157560] |