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碳酸酐酶Ⅷ(CA8)活性蛋白

Active Carbonic Anhydrase VIII (CA8)

CAVIII; CA-VIII; CALS; CARP; Carbonic anhydrase-related protein

  • 碳酸酐酶Ⅷ(CA8)活性蛋白 产品包装(模拟)
  • 碳酸酐酶Ⅷ(CA8)活性蛋白 产品包装(模拟)
  • APD075Hu01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2  H2O = HCO3-  H , which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA8, also called CArelated protein (CARP), is a cytosolic protein without CA activity (i.e., the reversible hydration of CO2) due to point mutations in the zinc-binding site. CA8 is expressed exclusively in Purkinje cells of the cerebellum, where it binds inositol 1,4,5-triphosphate receptor type 1. CA8 overexpression in human colorectal cancer and non-small cell lung cancer indicates that it plays a role in the process of invasion in these types of malignancy. The activity of recombinant human CA8 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 μL of various concentrations of CA8 (diluted by assay buffer) to 50 µL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37℃ for 5min, then read at a wavelength of 400 nm.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
APD075Hu01 碳酸酐酶Ⅷ(CA8)活性蛋白 Cell culture; Activity Assays.
RPD075Hu01 碳酸酐酶Ⅷ(CA8)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAD075Hu01 碳酸酐酶Ⅷ(CA8)多克隆抗体 WB; IHC; ICC; IP.
LMD075Hu 碳酸酐酶Ⅷ(CA8)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
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