羧酸酯酶1(CES1)活性蛋白
Active Carboxylesterase 1 (CES1)
ACAT; CEH; CES2; HMSE1; PCE1; SES1; TGH; Retinyl ester hydrolase; Serine esterase 1; Triacylglycerol hydrolase; Egasyn; Cocaine carboxylesterase; Liver Carboxylesterase 1
- 编号APC374Mu61
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性状冻干粉
- 纯度> 90%
- 等电点5.9
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
carboxylesterase 1(CES1) also known as Liver carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant mouse CES1 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50 mM Tris, 150 mM NaCl, pH 7.5 (Assay Buffer), ainitiated by addition 50 μL of various concentrations of CES1 (diluted by Assay Buffer) to 50 µL of 2 mM Substrate 4-NPA (100 mM stock in Acetone, diluted by Assay Buffer). Incubated at 37℃ for 10min, then read at a wavelength of 400 nm.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| APC374Mu02 | 羧酸酯酶1(CES1)活性蛋白 | Cell culture; Activity Assays. |
| APC374Mu61 | 羧酸酯酶1(CES1)活性蛋白 | Cell culture; Activity Assays. |
| EPC374Mu61 | 羧酸酯酶1(CES1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC374Mu02 | 羧酸酯酶1(CES1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC374Mu01 | 羧酸酯酶1(CES1)活性蛋白 | Cell culture; Activity Assays. |
| RPC374Mu01 | 羧酸酯酶1(CES1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC374Mu01 | 羧酸酯酶1(CES1)多克隆抗体 | WB; IHC; ICC; IP. |
参考文献
| 杂志 | 参考文献 |
| International Journal of Nanomedicine | Gold Nanoparticles Perturb Drug-Metabolizing Enzymes and Antioxidants in the Livers of Male Rats: Potential Impact on Drug Interactions [Pubmed: 32764932] |