羧酸酯酶2(CES2)活性蛋白
Active Carboxylesterase 2 (CES2)
CE-2; CES2A1; PCE-2; iCE; Intestine,Liver; Methylumbelliferyl-acetate deacetylase 2; Cocaine esterase
- 编号APF282Hu61
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性状冻干粉
- 纯度> 95%
- 等电点5.3
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 2016 ¥ 5040 ¥ 10080 ¥ 30240 ¥ 75600
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
SDS-PAGE图
-
通过ISO 9001、ISO 13485质量体系认证
活性实验
Carboxylesterase 2 (CES2) is a member of serine esterase family which can hydrolyze ester and amide bonds. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant human CES2 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50 mM Tris, 150 mM NaCl, pH 7.5 (Assay Buffer), ainitiated by addition 50 μL of various concentrations of CES2 (diluted by Assay Buffer) to 50 µL of 2 mM Substrate 4-NPA (100 mM stock in Acetone, diluted by Assay Buffer). Incubated at 37℃ for 10 min, then read at a wavelength of 400 nm.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPF282Hu01 | 羧酸酯酶2(CES2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APF282Hu61 | 羧酸酯酶2(CES2)活性蛋白 | Cell culture; Activity Assays. |
| EPF282Hu61 | 羧酸酯酶2(CES2)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| WEF282Hu | 羧酸酯酶2(CES2)检测试剂盒(酶联免疫吸附试验法,宽范围) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEF282Hu | 羧酸酯酶2(CES2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMF282Hu | 羧酸酯酶2(CES2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Metallomics | Comprehensive and comparative exploration of the Atp7b−/− mouse plasma proteome [Pubmed: 31815268] |