组织蛋白酶G(CTSG)活性蛋白
Active Cathepsin G (CTSG)
CG; CTS-G
- 编号APD993Ra01
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 95%
- 等电点11.2
- 应用Cell culture; Activity Assays.
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- 规格 10µg50µg 200µg 1mg 5mg
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活性实验
Cathepsin G (CTSG) is a neutral proteinase secreted by neutrophil granulocytes. It belongs to the family of serine proteases. Cathepsin G is a glycoprotein, which contains 1% carbohydrate. The activity assay of recombinant rat CTSG was measured by its ability to cleave the peptide substrate Suc-Ala-Ala Pro-Phe-pNA. The reaction was performed in 160 mM Tris-HCl, 1.6 M NaCl, pH 7.4 (Assay Buffer). The CTSG was diluted to different concentrations and the reaction was initiated by adding 50 ul CTSG to 50 ul 1 mM substrate and then read at 405 nm in kinetic mode for 5 minutes. The specific activity of recombinant rat CTSG is >70 pmol/min/µg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
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相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
APD993Ra01 | 组织蛋白酶G(CTSG)活性蛋白 | Cell culture; Activity Assays. |
RPD993Ra01 | 组织蛋白酶G(CTSG)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAD993Ra01 | 组织蛋白酶G(CTSG)多克隆抗体 | WB; IHC; ICC; IP. |
MAD993Ra21 | 组织蛋白酶G(CTSG)单克隆抗体 | WB; IHC; ICC; IP. |
SED993Ra | 组织蛋白酶G(CTSG)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMD993Ra | 组织蛋白酶G(CTSG)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
杂志 | 参考文献 |
journal of maternal-fetal & neonatal medicine | Cervical fluid calreticulin and cathepsin-G in pregnancies complicated by preterm prelabor rupture of membranes. [pubmed:28152632] |
Lung | Mass Spectrometry Analysis of the Exhaled Breath Condensate and Proposal of Dermcidin and S100A9 as Possible Markers for Lung Cancer Prognosis [Pubmed: 31115649] |