太阳集团tyc5997_平台(china)官方网站

趋化因子 (C-X-C基序)配体1(CXCL1)活性蛋白

Active Chemokine (C-X-C Motif) Ligand 1 (CXCL1)

NAP3; GRO1; GROa; GRO-A; MGSA-A; NAP3; SCYB1; Chemokine C-X-C-Motif Ligand 1; Melanoma Growth Stimulating Activity Alpha; Fibroblast Secretory Protein

  • 趋化因子 (C-X-C基序)配体1(CXCL1)活性蛋白 产品包装(模拟)
  • 趋化因子 (C-X-C基序)配体1(CXCL1)活性蛋白 产品包装(模拟)
  • APA041Mu01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Neutrophil Activating Protein 3 (NAP3) ,also known as chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family. NAP3 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, arteriogenesis, inflammation, wound healing, and tumorigenesis. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of NAP3 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension, 5×105 cells/ml in RPMI 1640 with FBS free) and recombinant human NAP3 (0.01ng/mL, 0.1ng/mL, 1ng/mL, 10ng/mL, 100ng/mL, 1000ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37℃ with 5% CO2 for 1h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×200) randomly (five fields for each filter). Result shows recombinant human NAP3 is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification(×100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification(×200). Statistical results were shown in Figure 2. The optimum chemotaxis of NAP3 occurs at 0.1~1ng/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
EPA041Mu61 趋化因子 (C-X-C基序)配体1(CXCL1)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA041Mu61 趋化因子 (C-X-C基序)配体1(CXCL1)活性蛋白 Cell culture; Activity Assays.
RPA041Mu02 趋化因子 (C-X-C基序)配体1(CXCL1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA041Mu03 趋化因子 (C-X-C基序)配体1(CXCL1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA041Mu01 趋化因子 (C-X-C基序)配体1(CXCL1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA041Mu01 趋化因子 (C-X-C基序)配体1(CXCL1)活性蛋白 Cell culture; Activity Assays.
APA041Mu02 趋化因子 (C-X-C基序)配体1(CXCL1)活性蛋白 Cell culture; Activity Assays.
PAA041Mu01 趋化因子 (C-X-C基序)配体1(CXCL1)多克隆抗体 WB; IHC; ICC; IP.
PAA041Mu02 趋化因子 (C-X-C基序)配体1(CXCL1)多克隆抗体 WB; IHC; ICC; IP.
PAA041Mu03 趋化因子 (C-X-C基序)配体1(CXCL1)多克隆抗体 WB; IHC; ICC; IP.
LAA041Mu71 趋化因子 (C-X-C基序)配体1(CXCL1)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA041Mu81 趋化因子 (C-X-C基序)配体1(CXCL1)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
SEA041Mu 趋化因子 (C-X-C基序)配体1(CXCL1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA041Mu 趋化因子 (C-X-C基序)配体1(CXCL1)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA041Mu 趋化因子 (C-X-C基序)配体1(CXCL1)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA041Mu 趋化因子 (C-X-C基序)配体1(CXCL1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA041Mu01 趋化因子 (C-X-C基序)配体1(CXCL1)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
中国眼耳鼻喉科杂志 白细胞介素10 上调表达对家兔慢性细菌性鼻窦炎黏膜创伤修复的影响 [article:11593]
Scientific Reports Epidermal CD147 expression plays a key role in IL-22-induced psoriatic dermatitis. [pubmed:28272440]
Phytotherapy Research Total flavonoids from sea buckthorn ameliorates lipopolysaccharide/cigarette smoke‐induced airway inflammation [Pubmed: 31209984]
Frontiers in Oncology CXCL1 as an Unfavorable Prognosis Factor Negatively Regulated by DACH1 in Non-small Cell Lung Cancer [Pubmed: 31998654]
Phytomedicine Baohuoside I suppresses breast cancer metastasis by downregulating the tumor-associated macrophages/CXC motif chemokine ligand 1 pathway [Pubmed: 32911383]
Lab Invest Extracellular cold-inducible RNA-binding protein regulates neutrophil extracellular trap formation and tissue damage in acute pancreatitis [Pubmed: 32709888]
Phytomedicine Aiduqing formula suppresses breast cancer metastasis via inhibiting CXCL1-mediated autophagy []
Cell Commun Signal Aiduqing formula inhibits breast cancer metastasis by suppressing TAM/CXCL1-induced Treg differentiation and infiltration [34461944]
Viruses Interleukin-10-Mediated Lymphopenia Caused by Acute Infection with Foot-and-Mouth Disease Virus in Mice [34960627]
留言咨询
Baidu
sogou