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趋化因子C-X3-C-基元配体1(CX3CL1)活性蛋白

Active Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)

NTN; ABCD3; C3Xkine; CXC3; CXC3C; NTT; SCYD1; ABCD3; FKN; Neurotactin; Fractalkine; Small Inducible Cytokine Subfamily D(Cys-X3-Cys)Member 1

  • 趋化因子C-X3-C-基元配体1(CX3CL1)活性蛋白 产品包装(模拟)
  • 趋化因子C-X3-C-基元配体1(CX3CL1)活性蛋白 产品包装(模拟)
  • APA040Hu01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) also known as fractalkine is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed.Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of CX3CL1 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (150ul cell suspension,106 cells/ml in RPMI 1640 with FBS free) and CX3CL1 (0.1ng/ml, 0.01ng/ml ,0.001ng/ml, 0.0001 and 0.00001ng/ml diluted separately in serum free RPMI 1640 ) was added in lower chamber with a polycarbonate filter(8um pore size) used to separate the two compartments. After incubation at 37℃ with 5% CO2 for 1h, the filter was removed,then cells in low chamber were observed by inverted microscope at low magnification (×10) and the number of migrated cells were counted using Fluorescence Activating Cell Sorter. Result shows CX3CL1 is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification(×10) were shown in Figure 1. Statistical results of FACS were shown in Figure 2. The optimum chemotaxis of CX3CL1 occurs at 0.001-0.00001ng/ml.

Figure 2.The chemotactic effect of CX3CL1 on THP-1 cells

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA040Hu01 趋化因子C-X3-C-基元配体1(CX3CL1)活性蛋白 Cell culture; Activity Assays.
RPA040Hu02 趋化因子C-X3-C-基元配体1(CX3CL1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA040Hu01 趋化因子C-X3-C-基元配体1(CX3CL1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA040Hu02 趋化因子C-X3-C-基元配体1(CX3CL1)活性蛋白 Cell culture; Activity Assays.
PAA040Hu01 趋化因子C-X3-C-基元配体1(CX3CL1)多克隆抗体 WB; IHC; ICC; IP.
PAA040Hu02 趋化因子C-X3-C-基元配体1(CX3CL1)多克隆抗体 WB; IHC; ICC; IP.
LAA040Hu81 趋化因子C-X3-C-基元配体1(CX3CL1)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA040Hu71 趋化因子C-X3-C-基元配体1(CX3CL1)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA040Hu22 趋化因子C-X3-C-基元配体1(CX3CL1)单克隆抗体 WB; IHC; ICC; IP.
SEA040Hu 趋化因子C-X3-C-基元配体1(CX3CL1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA040Hu 趋化因子C-X3-C-基元配体1(CX3CL1)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA040Hu 趋化因子C-X3-C-基元配体1(CX3CL1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA040Hu01 趋化因子C-X3-C-基元配体1(CX3CL1)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
World Journal of Gastroenterology Preliminary study correlating CX3CL1/CX3CR1 expression with gastric carcinoma and gastric carcinoma perineural invasion [NCBI: PMC3989981]
International Journal of Colorectal Disease Downregulation of CX3CR1 ameliorates experimental colitis: evidence for CX3CL1-CX3CR1-mediated immune cell recruitment. [pubmed:27942903]
Frontiers in Cellular and Infection Microbiology Reduced CX3CL1 Secretion Contributes to the Susceptibility of Oral Leukoplakia-Associated Fibroblasts to Candida albicans. [pubmed:27891323]
Blood Coagul Fibrinolysis Low shear stress upregulates the expression of fractalkine through the activation of mitogen-activated protein kinases in endothelial cells [Pubmed:29406386]
Journal of Neuroinflammation The prenatal challenge with lipopolysaccharide and polyinosinic: polycytidylic acid disrupts CX3CL1-CX3CR1 and CD200-CD200R signalling in the brains of … [Pubmed: 32829711]
Cells Maternal Immune Activation Sensitizes Male Offspring Rats to Lipopolysaccharide-Induced Microglial Deficits Involving the Dysfunction of CD200–CD200R and … [Pubmed: 32664639]
Journal of Neuroinflammation CX3CR1-microglia mediates neuroinflammation and blood pressure regulation in the nucleus tractus solitarii of fructose-induced hypertensive rats [Pubmed: 32532282]
JOURNAL OF PERIODONTAL RESEARCH Potential biomarkers reflecting inflammation in patients with severe periodontitis: Fractalkine (CX3CL1) and its receptor (CX3CR1) [33641164]
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