CD7分子(CD7)活性蛋白
Active Cluster Of Differentiation 7 (CD7)
GP40; LEU9; TP41; Tp40; p41 Protein; T-cell Antigen CD7; T-cell Leukemia Antigen; T-cell surface antigen Leu-9
- 编号APB784Hu61
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 缓冲液成份PBS, pH7.4, containing 5% Trehalose.
- 性状冻干粉
- 纯度> 95%
- 等电点4.8
- 应用Cell culture; Activity Assays.
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- 规格 10µg50µg 200µg 1mg 5mg
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活性实验
Cluster Of Differentiation 7 (CD7) is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development. SECTM1 has been proposed as a ligand for CD7. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CD7 and recombinant human SECTM1. Briefly, biotin-linked CD7 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to SECTM1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of CD7 and SECTM1 was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPB784Hu01 | CD7分子(CD7)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB784Hu61 | CD7分子(CD7)活性蛋白 | Cell culture; Activity Assays. |
RPB784Hu02 | CD7分子(CD7)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPB784Hu61 | CD7分子(CD7)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB784Hu01 | CD7分子(CD7)多克隆抗体 | WB; IHC; ICC; IP. |
MAB784Hu22 | CD7分子(CD7)单克隆抗体 | WB; IHC; ICC; IP. |
MAB784Hu25 | CD7分子(CD7)单克隆抗体 | WB; IHC; ICC; IP. |
MAB784Hu21 | CD7分子(CD7)单克隆抗体 | WB; IHC; ICC; IP. |
MAB784Hu23 | CD7分子(CD7)单克隆抗体 | WB; IHC; ICC; IP. |
MAB784Hu24 | CD7分子(CD7)单克隆抗体 | WB. |