冷诱导RNA结合蛋白(CIRBP)活性蛋白
Active Cold Inducible RNA Binding Protein (CIRBP)
CIRP; A18HNRNP; Glycine-Rich RNA Binding Protein
- 编号APG886Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 95%
- 等电点9.7
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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WB图
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通过ISO 9001、ISO 13485质量体系认证
活性实验
CIRBP (Cold-inducible RNA-binding protein) is considered to play a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival and act as a translational activator. Besides, ATXN1 (Ataxin-1) has been proven as an interactor of CIRBP. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human CIRBP and recombinant human ATXN1. Briefly, CIRBP were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ATXN1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CIRBP mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CIRBP and ATXN1 was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPG886Hu01 | 冷诱导RNA结合蛋白(CIRBP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APG886Hu01 | 冷诱导RNA结合蛋白(CIRBP)活性蛋白 | Cell culture; Activity Assays. |
| PAG886Hu01 | 冷诱导RNA结合蛋白(CIRBP)多克隆抗体 | WB,IHC,ICC/IF |
| MAG886Hu21 | 冷诱导RNA结合蛋白(CIRBP)单克隆抗体 | WB; IHC; ICC; IP. |
| SEG886Hu | 冷诱导RNA结合蛋白(CIRBP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMG886Hu | 冷诱导RNA结合蛋白(CIRBP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| CGG886Hu01 | 冷诱导RNA结合蛋白(CIRBP)cDNA | PCR、蛋白表达等 |
参考文献
| 杂志 | 参考文献 |
| J Cell Mol Med | Normothermic machine perfusion attenuates hepatic ischaemia‐reperfusion injury by inhibiting CIRP‐mediated oxidative stress and mitochondrial fission [34786826] |