冷诱导RNA结合蛋白(CIRBP)活性蛋白
Active Cold Inducible RNA Binding Protein (CIRBP)
CIRP; A18HNRNP; Glycine-Rich RNA Binding Protein
- 编号APG886Mu01
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 90%
- 等电点9.6
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1606 ¥ 4014 ¥ 8028 ¥ 24084 ¥ 60210
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产品包装(模拟)
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产品包装(模拟)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE
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Figure. Western Blot
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Cold-inducible RNA-binding protein (CIRBP) plays a critical role in controlling the cellular response upon confronting a variety of cellular stresses, including short wavelength ultraviolet light, hypoxia, and hypothermia. Besides, Heterogeneous Nuclear Ribonucleoprotein A1 (HNRPA1) has been identified as an interactor of CIRBP, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse CIRBP and recombinant mouse HNRPA1. Briefly, CIRBP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to HNRPA1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CIRBP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CIRBP and HNRPA1 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of CIRBP with HNRPA1.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| APG886Mu01 | 冷诱导RNA结合蛋白(CIRBP)活性蛋白 | Cell culture; Activity Assays. |
| RPG886Mu01 | 冷诱导RNA结合蛋白(CIRBP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| SEG886Mu | 冷诱导RNA结合蛋白(CIRBP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| J Cell Mol Med | Normothermic machine perfusion attenuates hepatic ischaemia‐reperfusion injury by inhibiting CIRP‐mediated oxidative stress and mitochondrial fission [34786826] |