粒细胞巨噬细胞集落刺激因子(GM-CSF)活性蛋白
Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF)
CSF2; GMCSF; Sargramostim; Molgramostin; Granulocyte-Macrophage Colony Stimulating Factor
- 编号APA045Hu61
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性状冻干粉
- 纯度> 95%
- 等电点5.2
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
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活性实验
TF-1, the human erythroleukemia cell line,provides a good system for detecting the activity of GM-CSF for it is a cell line of immature erythroid origin that completely depends on interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for long term growth. As reported, GM-CSF was also able to induce differentiation of human monoblastic leukemia cell line U937. In house data was obtained by the following experiment: TF-1 cells and U937 cells were incubated in the presence of various concentrations of rhGM-CSF, then cells were observed by inverted microscope everyday .
Cell proliferation of TF1 cells after incubation with GM-CSF (10ng/mL) for 3 days was shown in Figure 1.
Cell differentiation of U937 cells after incubation with GM-CSF (10ng/mL) for 5 days was shown in Figure 2.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
EPA045Hu61 | 粒细胞巨噬细胞集落刺激因子(GM-CSF)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA045Hu61 | 粒细胞巨噬细胞集落刺激因子(GM-CSF)活性蛋白 | Cell culture; Activity Assays. |
APA045Hu01 | 粒细胞巨噬细胞集落刺激因子(GM-CSF)活性蛋白 | Cell culture; Activity Assays. |
RPA045Hu01 | 粒细胞巨噬细胞集落刺激因子(GM-CSF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA045Hu01 | 粒细胞巨噬细胞集落刺激因子(GM-CSF)多克隆抗体 | WB; IHC; ICC; IP. |
PAA045Hu06 | 粒细胞巨噬细胞集落刺激因子(GM-CSF)多克隆抗体 | WB; IHC; ICC; IP. |
FAA045Hu31 | 抗粒细胞巨噬细胞集落刺激因子(GM-CSF)多克隆抗体(别藻蓝蛋白/花青染料cy7标记) | FCM |
MAA045Hu22 | 粒细胞巨噬细胞集落刺激因子(GM-CSF)单克隆抗体 | WB; IHC; ICC; IP. |
RAA045Hu21 | 粒细胞巨噬细胞集落刺激因子(GM-CSF)重组抗体 | WB; IF; ICC; IHC; IP; FCM. |
SEA045Hu | 粒细胞巨噬细胞集落刺激因子(GM-CSF)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
AEA045Hu | 抗粒细胞巨噬细胞集落刺激因子抗体(Anti-GM-CSF)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
SCA045Hu | 粒细胞巨噬细胞集落刺激因子(GM-CSF)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA045Hu | 粒细胞巨噬细胞集落刺激因子(GM-CSF)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA045Hu01 | 粒细胞巨噬细胞集落刺激因子(GM-CSF)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Journal of Toxicology and Environmental Health | Effects of 900-MHz Microwave Radiation on γ-Ray-Induced Damage to Mouse Hematopoietic System [PubMed: 20391130] |
5 | Safety and efficacy of the peptide-based therapeutic vaccine for HIV-1, Vacc-4×: a phase 2 randomised, double-blind, placebo-controlled trial [Pubmed:24525316] |
Archives of Virology | Genetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central China [Pubmed:29305646] |
BioMed Research International | Activated Macrophages of Monocytic Origin Predominantly Express Proinflammatory Cytokine Genes, Whereas Kupffer Cells Predominantly Express Anti … [] |
bulletin of experimental biology and medicine | Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal Origin [Pubmed: 31183654] |
Exp Mol Pathol | The regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal?¡ [33285209] |
Vaccine | Co-delivery of PSMA antigen epitope and mGM-CSF with a cholera toxin-like chimeric protein suppressed prostate tumor growth via activating dendritic cells and?¡ [33612342] |