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粒细胞集落刺激因子(GCSF)活性蛋白

Active Colony Stimulating Factor 3, Granulocyte (GCSF)

CSF3; G-CSF; Granulocyte Colony Stimulating Factor; Pluripoietin; Filgrastim; Lenograstim

  • 粒细胞集落刺激因子(GCSF)活性蛋白 产品包装(模拟)
  • 粒细胞集落刺激因子(GCSF)活性蛋白 产品包装(模拟)
  • APA042Ra01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. The activity of G-CSF is usually measured by a cell proliferation assay using M-NFS60 mouse myelogenous leukemia lymphoblast cells. M-NFS60 cells were seeded into triplicate wells of 96-well plates at a density of 8,000 cells/well with 2% serum standard 1640 which contains various concentrations of recombinant rat G-CSF. After incubated for 3 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2-4 hours at 37 ℃. Proliferation of M-NFS-60 cells after incubation with G-CSF for 3 days observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant rat G-CSF for 3 days. The result was shown in Figure 2. It was obvious that G-CSF significantly increased cell viability of M-NFS-60 cells. The EC50 is 0.63 ng/ml

Figure 2. Cell proliferation of M-NFS-60 cells after stimulated with G-CSF.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
APA042Ra01 粒细胞集落刺激因子(GCSF)活性蛋白 Cell culture; Activity Assays.
APA042Ra61 粒细胞集落刺激因子(GCSF)活性蛋白 Cell culture; Activity Assays.
EPA042Ra61 粒细胞集落刺激因子(GCSF)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA042Ra01 粒细胞集落刺激因子(GCSF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA042Ra01 粒细胞集落刺激因子(GCSF)多克隆抗体 WB; IHC; ICC; IP.
MAA042Ra21 粒细胞集落刺激因子(GCSF)单克隆抗体 WB; IHC; ICC; IP.
SEA042Ra 粒细胞集落刺激因子(GCSF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA042Ra 粒细胞集落刺激因子(GCSF)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA042Ra 粒细胞集落刺激因子(GCSF)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Scientific Reports The Transcriptional Foundations of Sp110-mediated Macrophage (RAW264. 7) Resistance to Mycobacterium tuberculosis H37Ra [Pubmed:26912204]
International Journal of Clinical and Experimental Pathology Low-density neutrophils in severe fever with thrombocytopenia syndrome (SFTS) display decreased function to phagocytose SFTS virus and enhanced capacity to synthesize cytokines [ISSN:1936-2625/IJCEP0045334]
International Immunopharmacology Ginsenoside Rg3 improves cyclophosphamide-induced immunocompetence in Balb/c mice [Pubmed: 30974284]
Mol Immunol Oridonin alleviates kanamycin-related hearing loss by inhibiting NLRP3/caspase-1/gasdermin D-induced inflammasome activation and hair cell pyroptosis [Pubmed:35749835]
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