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补体成分5a(C5a)活性蛋白

Active Complement Component 5a (C5a)

  • 补体成分5a(C5a)活性蛋白 产品包装(模拟)
  • 补体成分5a(C5a)活性蛋白 产品包装(模拟)
  • APA388Mu62.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Complement Component 5a (C5a) is a component of the complement system which plays a key role in promoting migration and adherence of neutrophils and monocytes to vessel walls. The activity of recombinant human C5a was also measured by its ability to induce N-acetyl-β-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. 3.2*106 differentiated U937 cells were added to the 24-well plate and different concentrations of rhC5a was added to the 24-well plate and incubated at 37 ℃ for 3min. The cells were centrifuged at 400 g for 3min, and the supernatant contained N-acetyl-β-D-glucosaminidase. The enzyme activity of N-acetyl-β-D-glucosaminidase was measured by the substrate of 4-Nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside. The result was shown in figure 1, It was obvious that rhC5a can significantly induce N-acetyl-β-D-glucosaminidase release.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA388Mu01 补体成分5a(C5a)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA388Mu62 补体成分5a(C5a)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA388Mu61 补体成分5a(C5a)活性蛋白 Cell culture; Activity Assays.
APA388Mu62 补体成分5a(C5a)活性蛋白 Cell culture; Activity Assays.
EPA388Mu61 补体成分5a(C5a)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA388Mu01 补体成分5a(C5a)多克隆抗体 WB; IHC; ICC; IP.
LAA388Mu71 补体成分5a(C5a)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA388Mu21 补体成分5a(C5a)单克隆抗体 WB; IHC; ICC; IP.
LAA388Mu72 补体成分5a(C5a)单克隆抗体(生物素标记) WB; IHC; ICC.
SEA388Mu 补体成分5a(C5a)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA388Mu 补体成分5a(C5a)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA388Mu 补体成分5a(C5a)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA388Mu 补体成分5a(C5a)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA388Mu 补体成分5a(C5a)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
PSA388Mu01 补体成分5a(C5a)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA388Mu01 补体成分5a(C5a)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Molecular Immunology Cathepsin D is released after severe tissue trauma in vivo and is capable of generating C5a in vitro [ScienceDirect: S0161589011008297]
Journal of Reproductive Immunology Elevated complement factor C5a in maternal and umbilical cord plasma in preeclampsia [PubMed: 23415845]
The Journal of Clinical Investigation Complement component 5 contributes to poor disease outcome in humans and mice with pneumococcal meningitis [PubMed: PMC3195471]
Journal of Antimicrobial Chemotherapy Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model [Pubmed: 23645587]
Journal of Orthopaedic Research Conversion from external fixator to intramedullary nail causes a second hit and impairs fracture healing in a severe trauma model [Pubmed: 23070742]
SpringerPlus Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in rats [Pubmed: 24353977]
American Journal fo Reproductive Immunology Evaluation of the Markers of Inflammation in the Umbilical Cord Blood of Newborns of Mothers with Thrombophilia [Pubmed:25209155]
Gynecol Obstet Invest Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia? [PubMed: 26485247]
Journal of Neuroimmunology Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis [Pubmed:27235358]
International Immunopharmacology Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery [Pubmed:26802602]
Digital Repository Dosagem de frações ativadas do sistema complemento em empiema induzido em ratos [10183]
Environmental Pollution Cadmium-induced immune abnormality is a key pathogenic event in human and rat models of preeclampsia [pubmed:27511439]
Cancer Letters Complement C5a/C5aR pathway potentiates the pathogenesis Q5 of gastric cancer by down-regulating p21 expression [pubmed:29031586]
Acta Paediatrica Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese children [DOI: 10.1111/apa.14106]
Scientific Reports Splenectomy modulates early immuno-inflammatory responses to trauma-hemorrhage and protects mice against secondary sepsis [Pubmed: 30291296]
Nutrients The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in Triathletes [Pubmed: 30736479]
Journal of NeuroVirology Intrathecal complement activation by the classical pathway in tick-borne encephalitis [Pubmed: 30850976]
PLoS One Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model [Pubmed: 31247004]
Frontiers in Immunology C5a/C5aR Pathway is Critical for the Pathogenesis of Psoriasis [Pubmed: 31447855]
Phytomedicine Novel evidence that an alternative complement cascade pathway is involved in optimal mobilization of hematopoietic stem/progenitor cells in Nlrp3 … []
Aging C5aR deficiency attenuates the breast cancer development via the p38/p21 axis [Pubmed: 32669478]
STEM CELL REVIEWS AND REPORTS A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem … [Pubmed: 32406006]
Modulation of C5a-C5aR interactions against murine mammary cancer cell line []
FASEB J C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease [Pubmed:35503088]
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