补体成分5a(C5a)活性蛋白
Active Complement Component 5a (C5a)
-
产品包装(模拟)
-
产品包装(模拟)
-
Figure. SDS-PAGE
-
通过ISO 9001、ISO 13485质量体系认证
活性实验
Complement Component 5a (C5a) is a component of the complement system which plays a key role in promoting migration and adherence of neutrophils and monocytes to vessel walls. The activity of recombinant human C5a was also measured by its ability to induce N-acetyl-β-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. 3.2*106 differentiated U937 cells were added to the 24-well plate and different concentrations of rhC5a was added to the 24-well plate and incubated at 37 ℃ for 3min. The cells were centrifuged at 400 g for 3min, and the supernatant contained N-acetyl-β-D-glucosaminidase. The enzyme activity of N-acetyl-β-D-glucosaminidase was measured by the substrate of 4-Nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside. The result was shown in figure 1, It was obvious that rhC5a can significantly induce N-acetyl-β-D-glucosaminidase release.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA388Mu01 | 补体成分5a(C5a)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA388Mu62 | 补体成分5a(C5a)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA388Mu61 | 补体成分5a(C5a)活性蛋白 | Cell culture; Activity Assays. |
| APA388Mu62 | 补体成分5a(C5a)活性蛋白 | Cell culture; Activity Assays. |
| EPA388Mu61 | 补体成分5a(C5a)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA388Mu01 | 补体成分5a(C5a)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA388Mu71 | 补体成分5a(C5a)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA388Mu21 | 补体成分5a(C5a)单克隆抗体 | WB; IHC; ICC; IP. |
| LAA388Mu72 | 补体成分5a(C5a)单克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEA388Mu | 补体成分5a(C5a)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA388Mu | 补体成分5a(C5a)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA388Mu | 补体成分5a(C5a)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA388Mu | 补体成分5a(C5a)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA388Mu | 补体成分5a(C5a)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PSA388Mu01 | 补体成分5a(C5a)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA388Mu01 | 补体成分5a(C5a)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Molecular Immunology | Cathepsin D is released after severe tissue trauma in vivo and is capable of generating C5a in vitro [ScienceDirect: S0161589011008297] |
| Journal of Reproductive Immunology | Elevated complement factor C5a in maternal and umbilical cord plasma in preeclampsia [PubMed: 23415845] |
| The Journal of Clinical Investigation | Complement component 5 contributes to poor disease outcome in humans and mice with pneumococcal meningitis [PubMed: PMC3195471] |
| Journal of Antimicrobial Chemotherapy | Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model [Pubmed: 23645587] |
| Journal of Orthopaedic Research | Conversion from external fixator to intramedullary nail causes a second hit and impairs fracture healing in a severe trauma model [Pubmed: 23070742] |
| SpringerPlus | Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in rats [Pubmed: 24353977] |
| American Journal fo Reproductive Immunology | Evaluation of the Markers of Inflammation in the Umbilical Cord Blood of Newborns of Mothers with Thrombophilia [Pubmed:25209155] |
| Gynecol Obstet Invest | Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia? [PubMed: 26485247] |
| Journal of Neuroimmunology | Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis [Pubmed:27235358] |
| International Immunopharmacology | Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery [Pubmed:26802602] |
| Digital Repository | Dosagem de frações ativadas do sistema complemento em empiema induzido em ratos [10183] |
| Environmental Pollution | Cadmium-induced immune abnormality is a key pathogenic event in human and rat models of preeclampsia [pubmed:27511439] |
| Cancer Letters | Complement C5a/C5aR pathway potentiates the pathogenesis Q5 of gastric cancer by down-regulating p21 expression [pubmed:29031586] |
| Acta Paediatrica | Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese children [DOI: 10.1111/apa.14106] |
| Scientific Reports | Splenectomy modulates early immuno-inflammatory responses to trauma-hemorrhage and protects mice against secondary sepsis [Pubmed: 30291296] |
| Nutrients | The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in Triathletes [Pubmed: 30736479] |
| Journal of NeuroVirology | Intrathecal complement activation by the classical pathway in tick-borne encephalitis [Pubmed: 30850976] |
| PLoS One | Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model [Pubmed: 31247004] |
| Frontiers in Immunology | C5a/C5aR Pathway is Critical for the Pathogenesis of Psoriasis [Pubmed: 31447855] |
| Phytomedicine | Novel evidence that an alternative complement cascade pathway is involved in optimal mobilization of hematopoietic stem/progenitor cells in Nlrp3 … [] |
| Aging | C5aR deficiency attenuates the breast cancer development via the p38/p21 axis [Pubmed: 32669478] |
| STEM CELL REVIEWS AND REPORTS | A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem … [Pubmed: 32406006] |
| Modulation of C5a-C5aR interactions against murine mammary cancer cell line [] | |
| FASEB J | C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease [Pubmed:35503088] |
留言咨询