补体因子B(CFB)活性蛋白
Active Complement Factor B (CFB)
CF-B; BF; BFD; CFAB; GBG; H2-Bf; PBF2; B-Factor; Properdin; Properdin factor B; C3/C5 convertase; Glycine-rich beta glycoprotein
- 编号APC011Hu03
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 90%
- 等电点6.7
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1642 ¥ 4104 ¥ 8208 ¥ 24624 ¥ 61560
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产品包装(模拟)
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产品包装(模拟)
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Figure. Gene Sequencing (Extract)
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Figure. SDS-PAGE
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Figure. Western Blot
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Complement Factor B (CFB) a component of the alternative pathway of complement activation. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease that associates with C3b to form the alternative pathway C3 convertase. The method of functional assay of CFB was tested in hemolysis assays. Two-fold dilute the recombinant human CFB with 0.9% NaCl, 2mmol/L MgCl2 ,and then add same volume of 1% rabbit erythrocyte (RaE) in 8mmol/L EDTA, the negative control only without MgCl2. All the samples incubated at 37℃. After 3 hours later, take 10μL supernatant and 90μL TMB incubated at 37℃ for 5-10 minutes. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The results are shown in Figure 1. It was obvious that the minimal effective concentration of CFB is 0.3125μg/mL.(A) 1% rabbit erythrocyte (RaE) treated with 0.3125μg/mL CFB for 1h
(B) Negative control (1% RaE treated with 0.3125ug/mL CFB, 8mmol/L EDTA) without MgCl2.
Figure. The hemolysis effection of recombinant human CFB
Figure. The hemolysis activity of recombinant human CFB
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPC011Hu05 | 补体因子B(CFB)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC011Hu03 | 补体因子B(CFB)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC011Hu02 | 补体因子B(CFB)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPC011Hu61 | 补体因子B(CFB)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC011Hu04 | 补体因子B(CFB)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC011Hu01 | 补体因子B(CFB)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC011Hu03 | 补体因子B(CFB)活性蛋白 | Cell culture; Activity Assays. |
| PAC011Hu03 | 补体因子B(CFB)多克隆抗体 | WB; IHC |
| PAC011Hu02 | 补体因子B(CFB)多克隆抗体 | WB; IHC; ICC; IP. |
| PAC011Hu01 | 补体因子B(CFB)多克隆抗体 | WB; IHC |
| PAC011Hu04 | 补体因子B(CFB)多克隆抗体 | WB; IHC; ICC; IP. |
| LAC011Hu71 | 补体因子B(CFB)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAC011Hu22 | 补体因子B(CFB)单克隆抗体 | WB; IHC; ICC; IP. |
| WEC011Hu | 补体因子B(CFB)检测试剂盒(酶联免疫吸附试验法,宽范围) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEC011Hu | 补体因子B(CFB)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC011Hu | 补体因子B(CFB)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| BMC Nephrology | Complement in patients receiving maintenance hemodialysis: functional screening and quantitative analysis [BioMed: 14712369] |
| J. Proteome Res. | Identification and Confirmation of Differentially Expressed Fucosylated Glycoproteins in the Serum of Ovarian Cancer Patients Using a Lectin Array and LC–MS/MS [ACS: pr300330z] |
| Pediatr Int | Atypical hemolytic uremic syndrome: Korean pediatric series [PubMed: 25443527] |
| Journal of Neuroimmunology | Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis [Pubmed:27235358] |
| Biochemical and Biophysical Research Communications | Adipose tissue complement factor B promotes adipocyte maturation. [pubmed:29137982] |
| Journal of proteome research | Serum exosomes from newborn piglets restrict porcine epidemic diarrhea virus infection [] |
| journal of human genetics | Identification of susceptibility locus shared by IgA nephropathy and inflammatory bowel disease in a Chinese Han population [Pubmed: 31857673] |
| Nephrol Dial Transplant | Association between thrombotic microangiopathy and activated alternative complement pathway in malignant nephrosclerosis [33367879] |
| JOURNAL OF GENERAL VIROLOGY | Changes in complement alternative pathway components, factor B and factor H during dengue virus infection in the AG129 mouse [33410734] |
| J Proteome Res | Early Diagnostic Ability of Human Complement Factor B in Pancreatic Cancer Is Partly Linked to Its Potential Tumor-Promoting Role [34766501] |
| J Clin Med | A Three-Protein Panel to Support the Diagnosis of Sepsis in Children [Pubmed:35329889] |