补体因子B(CFB)活性蛋白
Active Complement Factor B (CFB)
CF-B; BF; BFD; CFAB; GBG; H2-Bf; PBF2; B-Factor; Properdin; Properdin factor B; C3/C5 convertase; Glycine-rich beta glycoprotein
- 编号APC011Mu03
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点5.1
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1783 ¥ 4458 ¥ 8916 ¥ 26748 ¥ 66870
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Complement factor B (CFB), a 95-kDa protein, is a crucial catalytic element of the alternative pathway (AP) of complement. After binding of CFB to C3b, activation of the AP depends on the proteolytic cleavage of CFB by factor D to generate the C3 convertase (C3bBb). The C3 convertase contains the catalytic subunit of CFB (Bb), the enzymatic site for the cleavage of a new molecule of C3 into C3b. Thus, a binding ELISA assay was conducted to detect the association of recombinant mouse CFB with recombinant mouse CFD. Briefly, biotin-linked recombinant mouse CFB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CFD-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant mouse CFB and recombinant mouse CFD was shown in Figure 1, the EC50 for this effect is 1.238 ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPC011Mu02 | 补体因子B(CFB)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC011Mu03 | 补体因子B(CFB)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC011Mu03 | 补体因子B(CFB)活性蛋白 | Cell culture; Activity Assays. |
| RPC011Mu01 | 补体因子B(CFB)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC011Mu01 | 补体因子B(CFB)活性蛋白 | Cell culture; Activity Assays. |
| PAC011Mu02 | 补体因子B(CFB)多克隆抗体 | WB; IHC; ICC; IP. |
| PAC011Mu01 | 补体因子B(CFB)多克隆抗体 | WB; IHC; ICC; IP. |
| LAC011Mu71 | 补体因子B(CFB)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAC011Mu72 | 补体因子B(CFB)单克隆抗体(生物素标记) | WB; IHC; ICC. |
| WEC011Mu | 补体因子B(CFB)检测试剂盒(酶联免疫吸附试验法,宽范围) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEC011Mu | 补体因子B(CFB)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC011Mu | 补体因子B(CFB)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| BMC Nephrology | Complement in patients receiving maintenance hemodialysis: functional screening and quantitative analysis [BioMed: 14712369] |
| J. Proteome Res. | Identification and Confirmation of Differentially Expressed Fucosylated Glycoproteins in the Serum of Ovarian Cancer Patients Using a Lectin Array and LC–MS/MS [ACS: pr300330z] |
| Pediatr Int | Atypical hemolytic uremic syndrome: Korean pediatric series [PubMed: 25443527] |
| Journal of Neuroimmunology | Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis [Pubmed:27235358] |
| Biochemical and Biophysical Research Communications | Adipose tissue complement factor B promotes adipocyte maturation. [pubmed:29137982] |
| Journal of proteome research | Serum exosomes from newborn piglets restrict porcine epidemic diarrhea virus infection [] |
| journal of human genetics | Identification of susceptibility locus shared by IgA nephropathy and inflammatory bowel disease in a Chinese Han population [Pubmed: 31857673] |
| Nephrol Dial Transplant | Association between thrombotic microangiopathy and activated alternative complement pathway in malignant nephrosclerosis [33367879] |
| JOURNAL OF GENERAL VIROLOGY | Changes in complement alternative pathway components, factor B and factor H during dengue virus infection in the AG129 mouse [33410734] |
| J Proteome Res | Early Diagnostic Ability of Human Complement Factor B in Pancreatic Cancer Is Partly Linked to Its Potential Tumor-Promoting Role [34766501] |
| J Clin Med | A Three-Protein Panel to Support the Diagnosis of Sepsis in Children [Pubmed:35329889] |