半胱氨酸蛋白酶抑制剂A(CSTA)活性蛋白
Active Cystatin A (CSTA)
Cys-A; STF1; STFA; Stefin A; Cystatin-AS
- 编号APA476Po01
- 物种Sus scrofa; Porcine (Pig,猪) 相同的名称,不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点5.8
- 应用Cell culture; Activity Assays.
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- 规格 10µg50µg 200µg 1mg 5mg
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活性实验
Cystatin A (CSTA) is a member of family 1 of the cystatin superfamily. Like Cystatin B, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L. For example, immunohistochemical analysis of Cystatin A and cathepsin L is a useful indicator for malignancy in human epidermal keratinocytes. The ratio of cathepsin B and Cystatin A can be used in the differential diagnosis and treatment of patients with prostate carcinoma. The activity of recombinant pig Cystatin A was measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC in the assay buffer 50 mM Tris, pH 7.0. Papain was diluted to 500 ug/ml in activation buffer 50 mM Tris, 5 mM DTT, pH 7.0 and incubated at room temperature for 15 minutes. The activated papain was diluted to 100 ug/ml in the assay buffer and 20 ul different concentrations of recombinant pig Cystatin A (MW: 40.9 KD) was incubated with 20 ul 100 ug/ml papain at 37 ℃ for 10 minutes. Loading 50 µL of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 µL of 200 µM substrate. Include a substrate blank containing 50 µL of assay buffer and 50 µL of 200 µM substrate. Then read at excitiation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant pig Cystatin A significantly decreased papain activity. The inhibition IC50 was <300 nM.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
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相关产品
编号 | 适用物种:Sus scrofa; Porcine (Pig,猪) | 应用(仅供研究使用,不用于临床诊断!) |
APA476Po01 | 半胱氨酸蛋白酶抑制剂A(CSTA)活性蛋白 | Cell culture; Activity Assays. |
RPA476Po01 | 半胱氨酸蛋白酶抑制剂A(CSTA)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA476Po01 | 半胱氨酸蛋白酶抑制剂A(CSTA)多克隆抗体 | WB; IHC; ICC; IP. |
参考文献
杂志 | 参考文献 |
Journal of Virology | Imiquimod Suppresses Propagation of Herpes Simplex Virus 1 by Upregulation of Cystatin A via the Adenosine Receptor A1 Pathway [Asm: Source] |
Scientific Reports | Increased levels of the megakaryocyte and platelet expressed cysteine proteases stefin A and cystatin A prevent thrombosis [Pubmed: 31270351] |
International Journal of Reproductive Medicine | Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis [33575317] |
Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … [Pubmed:35585377] |