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细胞色素P450家族成员1B1(CYP1B1)活性蛋白

Active Cytochrome P450 1B1 (CYP1B1)

CP1B; GLC3A; Cytochrome P450,Family 1,Subfamily B,Polypeptide 1; Glaucoma 3,Primary Infantile

  • 细胞色素P450家族成员1B1(CYP1B1)活性蛋白 产品包装(模拟)
  • 细胞色素P450家族成员1B1(CYP1B1)活性蛋白 产品包装(模拟)
  • APD297Hu01.jpg Figure. SDS-PAGE
  • 细胞色素P450家族成员1B1(CYP1B1)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Cytochrome P450 family 1 subfamily B member 1 (CYP1B1) is an enzyme, Which belongs to the cytochrome P450 superfamily. CYP1B1 can catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids. Besides, Heat Shock Protein family B (small) member 2 (HSPb2) has been identified as an interactor of CYP1B1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CYP1B1 and recombinant human HSPb2, CYP1B1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HSPb2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CYP1B1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CYP1B1 and HSPb2 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of CYP1B1 with HSPb2.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPD297Hu01 细胞色素P450家族成员1B1(CYP1B1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APD297Hu01 细胞色素P450家族成员1B1(CYP1B1)活性蛋白 Cell culture; Activity Assays.
PAD297Hu01 细胞色素P450家族成员1B1(CYP1B1)多克隆抗体 WB; IHC; ICC; IP.
LAD297Hu71 细胞色素P450家族成员1B1(CYP1B1)多克隆抗体(生物素标记) WB; IHC; ICC.
MAD297Hu21 细胞色素P450家族成员1B1(CYP1B1)单克隆抗体 WB; IHC; ICC; IP.
SED297Hu 细胞色素P450家族成员1B1(CYP1B1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD297Hu 细胞色素P450家族成员1B1(CYP1B1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Int J Mol Sci Lipidomic Profile and Enzymes Activity in Hepatic Microsomes of Rats in Physiological and Pathological Conditions [Pubmed:35008866]
ResearchSquare Effects of herbicide atrazine on reproductive hormonal levels, Cytochrome P450, and gonadal structure of adult male and female crayfish, Procambarus clarkii []
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