内分泌腺来源血管内皮生长因子(EG-VEGF)活性蛋白
Active Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF)
PK1; EGVEGF; PRK1, PROK1; Prokineticin 1; Black Mamba Toxin-Related Protein; Mambakine
- 编号APA024Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 90%
- 等电点8.1
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1267 ¥ 3168 ¥ 6336 ¥ 19008 ¥ 47520
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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Figure. Western Blot
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Figure 1. Cell proliferation of ECV-304 cells after stimulated with EG-VEGF.
Endothelial gland-derived VEGF (EG-VEGF) is an angiogenic protein that is structurally unrelated to VEGF. It is expressed in steroidogenic tissues such as adrenal gland, ovary, testis, and placenta. Like VEGF it can induce fenestrae in endothelial cells. To test the effect of EG-VEGF on cell proliferation of ECV-304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of EG-VEGF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37℃.
(A) Unstimulated ECV-304 cells cultured in 1640 for 96h;
(B) ECV-304 cells cultured in 1640, stimulated with 10ng/mL VEGF121 for 96h.
Figure. Cell proliferation of ECV-304 cells after stimulated with EG-VEGF
用法
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA024Hu01 | 内分泌腺来源血管内皮生长因子(EG-VEGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA024Hu01 | 内分泌腺来源血管内皮生长因子(EG-VEGF)活性蛋白 | Cell culture; Activity Assays. |
| PAA024Hu01 | 内分泌腺来源血管内皮生长因子(EG-VEGF)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA024Hu22 | 内分泌腺来源血管内皮生长因子(EG-VEGF)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA024Hu | 内分泌腺来源血管内皮生长因子(EG-VEGF)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA024Hu | 内分泌腺来源血管内皮生长因子(EG-VEGF)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA024Hu | 内分泌腺来源血管内皮生长因子(EG-VEGF)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Tumor Biology | Hypoxia-induced secretion of IL-14 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151] |
| Stem Cells and Development | Lower Senescence of Adipose-Derived Stem Cells than Donor-Matched Bone Marrow Stem Cells for Surgical Ventricular Restoration [Pubmed:29630447] |