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内皮抑素(ES)活性蛋白

Active Endostatin (ES)

  • 内皮抑素(ES)活性蛋白 产品包装(模拟)
  • 内皮抑素(ES)活性蛋白 产品包装(模拟)
  • APA542Hu61.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Endostatin (ES) is a naturally occurring, 20kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. Endostatin is a broad-spectrum angiogenesis inhibitor and may interfere with the pro-angiogenic action of growth factors such as basic fibroblast growth factor (bFGF/FGF-2) and vascular endothelial growth factor (VEGF). To test the effect of ES on inhibit the VEGF basic-dependent proliferation of ECV304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 5, 000 cells/well when the cell attached, replaced with serum-free standard DMEM overnight. Then the medium was replaced with 2% serum standard DMEM which contain 10ng/mL of VEGFA and various concentrations of ES. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37℃. The result was shown in Figure 1. It was obvious that ES (100ng/mL) significantly inhibit cell proliferation of EV304 cells.

用法

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA542Hu61 内皮抑素(ES)活性蛋白 Cell culture; Activity Assays.
EPA542Hu61 内皮抑素(ES)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPA542Hu31 内皮抑素(ES)钥孔血蓝蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA542Hu01 内皮抑素(ES)多克隆抗体 WB; IHC; ICC; IP.
PAA542Hu06 内皮抑素(ES)多克隆抗体 WB; IHC; ICC; IP.
WEA542Hu 内皮抑素(ES)检测试剂盒(酶联免疫吸附试验法,宽范围) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA542Hu 内皮抑素(ES)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA542Hu 内皮抑素(ES)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA542Hu 内皮抑素(ES)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA542Hu11 内皮抑素(ES)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Cancer Immunology Endostatin gene therapy enhances the efficacy of IL-2 in suppressing metastatic renal cell carcinoma in mice [SpringerLink: u38777121t6660l5]
Journal of Fluoresence Erythrocyte Protoporphyrin Fluorescence as a Biomarker for Monitoring Antiangiogenic Cancer Therapy [SpringerLink: 4v306130302257w0]
The Journal of Gene Medicine Endostatin- and interleukin-2-expressing retroviral bicistronic vector for gene therapy of metastatic renal cell carcinoma [Wiley: source]
Biomedicine & Pharmacotherapy Endostatin neoadjuvant gene therapy extends survival in an orthotopic metastatic mouse model of renal cell carcinoma [ScienceDirect: S075333221100151X]
Science Alert Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in Rats [Scialert: Source]
The Journal of Immunology Inflammation Induced by MMP-9 Enhances Tumor Regression of Experimental Breast Cancer [PubMed: PMC3619527]
Prostaglandins & Other Lipid Mediators Promotion of adipogenesis by an EP2 receptor agonist via stimulation of angiogenesis in pulmonary emphysema [Pubmed:24911647]
American Journal of Physiology - Heart and Circulatory Physiology Endostatin and kidney fibrosis in aging: a case for antagonistic pleiotropy? [Source]
shock Angiogenesis inhibitor endostatin protects mice with sepsis from multiple organ dysfunction syndrome [PubMed: 26125086]
Clin Rev Allergy Immunol Endothelial Dysfunction and Nailfold Videocapillaroscopy Pattern as Predictors of Digital Ulcers in Systemic Sclerosis: a Cohort Study and Review of the Literature [PubMed: 26142066]
Angiologia e Cirurgia Vascular Peripheral vasculopathy in Raynaud phenomenon: Vascular disease biomarkers [science:S1646706X16300052]
Clinical Rheumatology Impaired angiogenesis as a feature of digital ulcers in systemic sclerosis [Pubmed:26920752]
Blood biomarker for early blood brain barrier disruption in ischemic stroke [patent:US20170199205A1]
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