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成纤维细胞生长因子10(FGF10)活性蛋白

Active Fibroblast Growth Factor 10 (FGF10)

Keratinocyte growth factor 2

  • 成纤维细胞生长因子10(FGF10)活性蛋白 产品包装(模拟)
  • 成纤维细胞生长因子10(FGF10)活性蛋白 产品包装(模拟)
  • 成纤维细胞生长因子10(FGF10)活性蛋白 Figure . Gene Sequencing (extract)
  • APB882Hu01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Fibroblast Growth Factor 10(FGF10)is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Besides, Fibroblast Growth Factor Receptor 2 (FGFR2) has been identified as an interactor of FGF10, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF10 and recombinant human FGFR2. Briefly, FGF10 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to FGFR2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FGF10 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of FGF10 and FGFR2 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPB882Hu01 成纤维细胞生长因子10(FGF10)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPB882Hu61 成纤维细胞生长因子10(FGF10)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB882Hu01 成纤维细胞生长因子10(FGF10)活性蛋白 Cell culture; Activity Assays.
PAB882Hu06 成纤维细胞生长因子10(FGF10)多克隆抗体 WB; IHC; ICC; IP.
PAB882Hu01 成纤维细胞生长因子10(FGF10)多克隆抗体 WB; IHC; ICC; IP.
MAB882Hu22 成纤维细胞生长因子10(FGF10)单克隆抗体 WB; IHC; ICC; IP.
SEB882Hu 成纤维细胞生长因子10(FGF10)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB882Hu 成纤维细胞生长因子10(FGF10)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMB882Hu 成纤维细胞生长因子10(FGF10)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Inflammation Involvements of γδT Lymphocytes in Acute and Chronic Skin Wound Repair. [pubmed:28540539]
Journal of Molecular Neuroscience Dysregulation of Fibroblast Growth Factor 10 in the Peripheral Blood of Patients with Schizophrenia [Pubmed: 31256336]
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