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成纤维细胞生长因子17(FGF17)活性蛋白

Active Fibroblast Growth Factor 17 (FGF17)

  • 成纤维细胞生长因子17(FGF17)活性蛋白 产品包装(模拟)
  • 成纤维细胞生长因子17(FGF17)活性蛋白 产品包装(模拟)
  • APC910Hu02.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Fibroblast growth factor 17 (FGF17) is a member of the fibroblast growth factorfamily. FGF family members possess broad mitogenicand cell survival activities and are involved in a varietyof biological processes, including embryonicdevelopment cell growth, morphogenesis, tissue repair, tumor growth, andinvasion. Fibroblast Growth Factor Receptor 1 (FGFR1) is high affinity receptor for FGF17, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human FGF17 and recombinant human FGFR1. Briefly, biotin-linked FGF17 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FGFR1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human FGF17 and human FGFR1 was shown in Figure 1, the EC50 for this effect is 0.52 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPC910Hu01 成纤维细胞生长因子17(FGF17)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC910Hu02 成纤维细胞生长因子17(FGF17)活性蛋白 Cell culture; Activity Assays.
RPC910Hu02 成纤维细胞生长因子17(FGF17)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC910Hu01 成纤维细胞生长因子17(FGF17)活性蛋白 Cell culture; Activity Assays.
PAC910Hu01 成纤维细胞生长因子17(FGF17)多克隆抗体 WB; IHC; ICC; IP.
MAC910Hu21 成纤维细胞生长因子17(FGF17)单克隆抗体 WB; IHC; ICC; IP.
SEC910Hu 成纤维细胞生长因子17(FGF17)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC910Hu 成纤维细胞生长因子17(FGF17)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
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