成纤维细胞生长因子18(FGF18)活性蛋白
Active Fibroblast Growth Factor 18 (FGF18)
ZFGF5
- 编号APC907Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 90%
- 等电点10.7
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1884 ¥ 4710 ¥ 9420 ¥ 28260 ¥ 70650
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
Figure . Gene Sequencing (extract)
-
Figure. SDS-PAGE
-
通过ISO 9001、ISO 13485质量体系认证
活性实验
Fibroblast Growth Factor-18 (FGF18) is a trophic factor for mature chondrocytes
and their progenitors. It has been reported to have significant anabolic effects on
cartilage. FGF18 plays a central role in skeletal growth and
development.Besides,Fibroblast Growth Factor Receptor 3 (FGFR3) has been
identified as an interactor of FGF18, thus a binding ELISA assay was conducted
to detect the interaction of recombinant human FGF18 and recombinant human
FGFR3. Briefly, FGF18 were diluted serially in PBS, with 0.01% BSA (pH 7.4).
Duplicate samples of 100μl were then transferred to FGFR3-coated microtiter
wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated
for 1h with anti-FGF18 pAb, then aspirated and washed 3 times. After incubation
with HRP labelled secondary antibody, wells were aspirated and washed 3 times.
With the addition of substrate solution, wells were incubated 15-25 minutes at
37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately.
The binding activity of FGF18 and FGFR3 was shown in Figure 1, and this effect
was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| EPC907Hu62 | 成纤维细胞生长因子18(FGF18)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC907Hu02 | 成纤维细胞生长因子18(FGF18)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC907Hu02 | 成纤维细胞生长因子18(FGF18)活性蛋白 | Cell culture; Activity Assays. |
| RPC907Hu01 | 成纤维细胞生长因子18(FGF18)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC907Hu01 | 成纤维细胞生长因子18(FGF18)活性蛋白 | Cell culture; Activity Assays. |
| PAC907Hu01 | 成纤维细胞生长因子18(FGF18)多克隆抗体 | WB; IHC; ICC; IP. |
| MAC907Hu22 | 成纤维细胞生长因子18(FGF18)单克隆抗体 | WB; IHC; ICC; IP. |
| SEC907Hu | 成纤维细胞生长因子18(FGF18)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEC907Hu | 成纤维细胞生长因子18(FGF18)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCC907Hu | 成纤维细胞生长因子18(FGF18)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| CCC907Hu | 成纤维细胞生长因子18(FGF18)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMC907Hu | 成纤维细胞生长因子18(FGF18)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Pesqui. Bras. Odontopediatria Clín. Integr | Human Umbilical Cord Mesenchymal Stem Cells Over Platelet Rich Fibrin Scaffold for Mandibular Cartilage Defects Regenerative Medicine [] |
| J Exp Clin Cancer Res | HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway [Pubmed:35277183] |