成纤维细胞生长因子3(FGF3)活性蛋白
Active Fibroblast Growth Factor 3 (FGF3)
HBGF3; INT2; Heparin-binding growth factor 3; Murine Mammary Tumor Virus Integration Site(v-int-2)Oncogene Homolog; INT-2 Proto-Oncogene Protein; Proto-oncogene Int-2
- 编号APC906Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点11.0
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1267 ¥ 3168 ¥ 6336 ¥ 19008 ¥ 47520
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产品包装(模拟)
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产品包装(模拟)
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Figure. Gene Sequencing (Extract)
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Figure. SDS-PAGE
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WB图
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Fibroblast Growth Factor 3 (FGF3) also known as INT-2 proto-oncogene protein is a member of the fibroblast growth factor family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Besides, Fibroblast Growth Factor Receptor 4 (FGFR4) has been identified as an interactor of FGF3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF3 and recombinant human FGFR4. Briefly, biotin-linked FGF3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FGFR4-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human FGF3 and recombinant human FGFR4 was shown in Figure 1, the EC50 for this effect is 0.52 ug/mL.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPC906Hu01 | 成纤维细胞生长因子3(FGF3)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC906Hu01 | 成纤维细胞生长因子3(FGF3)活性蛋白 | Cell culture; Activity Assays. |
| RPC906Hu02 | 成纤维细胞生长因子3(FGF3)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC906Hu01 | 成纤维细胞生长因子3(FGF3)多克隆抗体 | WB; IHC; ICC; IP. |
| LAC906Hu71 | 成纤维细胞生长因子3(FGF3)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAC906Hu22 | 成纤维细胞生长因子3(FGF3)单克隆抗体 | WB; IHC; ICC; IP. |
| SEC906Hu | 成纤维细胞生长因子3(FGF3)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCC906Hu | 成纤维细胞生长因子3(FGF3)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMC906Hu | 成纤维细胞生长因子3(FGF3)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Mol Med Rep | Screening a novel FGF3 antagonist peptide with anti-tumor effects on breast cancer from a phage display library [PubMed: 26323695] |