半乳糖凝集素8(GAL8)活性蛋白
Active Galectin 8 (GAL8)
LGALS8; PCTA1; Po66-CBP; Prostate Carcinoma Tumor Antigen 1; Lectin,Galactoside-Binding Soluble 8; Po66 carbohydrate-binding protein; Prostate carcinoma tumor antigen 1
- 编号APA308Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 95%
- 等电点8.3
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1814 ¥ 4536 ¥ 9072 ¥ 27216 ¥ 68040
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Galectin 8 (GAL8), also known as prostate carcinoma tumor antigen 1 (PCTA1) in human, is a tandem repeat-type galectin. is a member of the lectin family, of which 14 mammalian galectins have been identified. It is also a member of the beta-galactoside-binding protein family that plays an important role in cell-cell adhesion, cell-matrix interactions, macrophage activation, angiogenesis, metastasis, apoptosis. In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant human GAL8 with 0.9% sodium chloride injection, add 50μL a serial dilution of GAL8 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control with no GAL8, replace with 50μL 0.9% sodium chloride injection. Then add 50μL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL8 is 6.25μg/mL.
Figure 2. The hemagglutination assay of GAL8 in V- bottom shaped 96-well microtiter plate.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA308Hu01 | 半乳糖凝集素8(GAL8)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA308Hu01 | 半乳糖凝集素8(GAL8)活性蛋白 | Cell culture; Activity Assays. |
| PAA308Hu01 | 半乳糖凝集素8(GAL8)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA308Hu71 | 半乳糖凝集素8(GAL8)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA308Hu81 | 半乳糖凝集素8(GAL8)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA308Hu27 | 半乳糖凝集素8(GAL8)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA308Hu25 | 半乳糖凝集素8(GAL8)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA308Hu24 | 半乳糖凝集素8(GAL8)单克隆抗体 | WB; ICC/IF |
| MAA308Hu29 | 半乳糖凝集素8(GAL8)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA308Hu23 | 半乳糖凝集素8(GAL8)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA308Hu21 | 半乳糖凝集素8(GAL8)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA308Hu22 | 半乳糖凝集素8(GAL8)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA308Hu | 半乳糖凝集素8(GAL8)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA308Hu | 半乳糖凝集素8(GAL8)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA308Hu01 | 半乳糖凝集素8(GAL8)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Cell Metabolism | Podoplanin-Expressing Macrophages Promote Lymphangiogenesis and Lymphoinvasion in Breast Cancer [Pubmed: 31447322] |
| CALCIFIED TISSUE INTERNATIONAL | The Dysregulated Galectin Network Activates NF-κB to Induce Disease Markers and Matrix Degeneration in 3D Pellet Cultures of Osteoarthritic Chondrocytes [Pubmed: 33185768] |