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高尔基磷酸化蛋白2(GOLPH2)活性蛋白

Active Golgi Phosphoprotein 2 (GOLPH2)

GOLM1; GP73; C9orf155; Golgi Membrane Protein 1; Golgi Protein 73

  • 高尔基磷酸化蛋白2(GOLPH2)活性蛋白 产品包装(模拟)
  • 高尔基磷酸化蛋白2(GOLPH2)活性蛋白 产品包装(模拟)
  • APB668Mu01.jpg SDS-PAGE图
  • 高尔基磷酸化蛋白2(GOLPH2)活性蛋白 Figure. Western Blot; Sample: Recombinant GP73, Mouse.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Golgi Protein 73 (GP73) also known as Golgi phosphoprotein 2 or Golgi membrane protein GP73 is a protein that encoded by the GOLM1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. It has been observed to be upregulated in response to viral infection. Besides, Dymeclin (DYM) has been identified as an interactor of GP73, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse GP73 and recombinant human DYM. Briefly, GP73 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to DYM-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GP73 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of GP73 and DYM was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
RPB668Mu02 高尔基磷酸化蛋白2(GOLPH2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB668Mu01 高尔基磷酸化蛋白2(GOLPH2)活性蛋白 Cell culture; Activity Assays.
RPB668Mu01 高尔基磷酸化蛋白2(GOLPH2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB668Mu02 高尔基磷酸化蛋白2(GOLPH2)多克隆抗体 WB; IHC; ICC; IP.
PAB668Mu01 高尔基磷酸化蛋白2(GOLPH2)多克隆抗体 WB; IHC; ICC; IP; FCM
LAB668Mu81 高尔基磷酸化蛋白2(GOLPH2)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAB668Mu71 高尔基磷酸化蛋白2(GOLPH2)多克隆抗体(生物素标记) WB; IHC; ICC.
SEB668Mu 高尔基磷酸化蛋白2(GOLPH2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB668Mu 高尔基磷酸化蛋白2(GOLPH2)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB668Mu01 高尔基磷酸化蛋白2(GOLPH2)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Molecular & Cellular Proteomics LAMC2: A promising new pancreatic cancer biomarker identified by proteomic analysis of pancreatic adenocarcinoma tissues [Mcponline: 023507]
Afro-Egypt J Infect Endem Dis Diagnostic and Prognostic Validity of Serum Golgi Protein 73 in Egyptian Patients with Hepatocelluar Carcinoma [Ajied: Ajied_System_Files]
Journal of Applied Pharmaceutical Science Diagnostic role of Golgi protein 73 and IL-17 in Egyptian cirrhotic rather than hepatocellular carcinoma patients. [handle:123456789]
Oncology Letters Serum Golgi protein 73 is a prognostic rather than diagnostic marker in hepatocellular carcinoma [pubmed:29113278]
Scientific Reports The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study [Pubmed: 32198440]
Hepatobiliary & Pancreatic Diseases International Dynamic expression of hepatic GP73 mRNA and protein and circulating GP73 during hepatocytes malignant transformation [Pubmed: 32171652]
Biomolecules Deoxycholic Acid Upregulates Serum Golgi Protein 73 through Activating NF-¦ÊB Pathway and Destroying Golgi Structure in Liver Disease. Biomolecules 2021, 11?¡­ [33540642]
ADVANCED RESEARCHES Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C []
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