颗粒酶K(GZMK)活性蛋白
Active Granzyme K (GZMK)
GZM-K; TRYP2; PRSS; Serine Protease,Granzyme 3; Tryptase II; Fragmentin-3; Granzyme-3; NK-tryptase-2
- 编号APB209Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点9.5
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1382 ¥ 3456 ¥ 6912 ¥ 20736 ¥ 51840
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Granzyme K is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Human granzyme K is synthesized as a precursor (264 residues) with a signal peptide (residues 1 24), a propeptide (residues 25-26) and a mature chain (residues 27-264). The purified recombinant human Granzyme K consists of residues 27 to 264 which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl•HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (assay buffer), initiated by addition 50 μL of various concentrations of GZMK (diluted by assay buffer) to 50 µL of 1.2 mM substrate and DTNB mixture. The final well serves as a negative control with no GZMK, replace with 50 μL assay buffer. Incubated at 25℃ for 5min, then read at a wavelength of 405 nm. The specific activity of recombinant human Granzyme K is >220 pmol/min/µg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB209Hu01 | 颗粒酶K(GZMK)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB209Hu01 | 颗粒酶K(GZMK)活性蛋白 | Cell culture; Activity Assays. |
| PAB209Hu01 | 颗粒酶K(GZMK)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB209Hu81 | 颗粒酶K(GZMK)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAB209Hu71 | 颗粒酶K(GZMK)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAB209Hu21 | 颗粒酶K(GZMK)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB209Hu22 | 颗粒酶K(GZMK)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB209Hu | 颗粒酶K(GZMK)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB209Hu | 颗粒酶K(GZMK)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Immunobiology. | Granzyme M and K release in human experimental endotoxemia [science:S0171298516300225] |