颗粒酶M(GZMM)活性蛋白
Active Granzyme M (GZMM)
GZM-M; LMET1; MET1; Hu-Met-1; Met-ase; Lymphocyte Met Ase 1; Met-1 serine protease; Natural killer cell granular protease
- 编号APA431Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 90%
- 等电点10.3
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1920 ¥ 4800 ¥ 9600 ¥ 28800 ¥ 72000
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产品包装(模拟)
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产品包装(模拟)
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Gene sequencing
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Figure. SDS-PAGE
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Figure. Western Blot; Sample: Recombinant GZMM, Human.
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通过ISO 9001、ISO 13485质量体系认证
活性实验
GZMM (Granzyme M) is one of the neutral serine proteases, which is specifically expressed by NK cells and mediates a novel major and perforin-dependent cell death pathway. Granzyme M has been proven to targets a-Tubulin and disorganizes the microtubule network, besides, Ezrin has also been identified as a substrate of GZMM. Human granzyme M is synthesized as a precursor (264 residues) with a signal peptide (residues 1 23), a propeptide (residues 24-25) and a mature chain (residues 26-257). The purified recombinant human Granzyme M consists of residues 26 to 257 which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl•HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (assay buffer), initiated by addition 50 μL of various concentrations of GZMM (diluted by assay buffer) to 50 µL of 1.2 mM substrate and DTNB mixture. The final well serves as a negative control with no GZMM, replace with 50 μL assay buffer. Incubated at 25℃ for 5min, then read at a wavelength of 405 nm. The specific activity of recombinant human Granzyme M is >80 pmol/min/µg.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA431Hu01 | 颗粒酶M(GZMM)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA431Hu01 | 颗粒酶M(GZMM)活性蛋白 | Cell culture; Activity Assays. |
| PAA431Hu01 | 颗粒酶M(GZMM)多克隆抗体 | WB; IHC |
| LAA431Hu71 | 颗粒酶M(GZMM)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA431Hu81 | 颗粒酶M(GZMM)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA431Hu23 | 颗粒酶M(GZMM)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA431Hu22 | 颗粒酶M(GZMM)单克隆抗体 | WB,IHC |
| SEA431Hu | 颗粒酶M(GZMM)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Clinical Immunology | Elevated granzyme M-expressing lymphocytes during cytomegalovirus latency and reactivation after allogeneic stem cell transplantation [ScienceDirect: S1521661613002970] |
| Immunobiology. | Granzyme M and K release in human experimental endotoxemia [science:S0171298516300225] |