生长分化因子9(GDF9)活性蛋白
Active Growth Differentiation Factor 9 (GDF9)
- 编号APA427Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 90%
- 等电点7.1
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1973 ¥ 4932 ¥ 9864 ¥ 29592 ¥ 73980
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产品包装(模拟)
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产品包装(模拟)
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SDS-PAGE图
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WB图
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通过ISO 9001、ISO 13485质量体系认证
活性实验
GDF9 (Growth/differentiation factor 9) is an oocyte derived growth factora which belongs to the transforming growth factor-beta (TGFβ) superfamily. GDF9 is required for ovarian folliculogenesis and promotes primordial follicle development. S100A8 has been identified as an interactor of GDF9 through two-hybrid assay, thus a binding ELISA assay was conducted to detect the interaction of recombinant human GDF9 and recombinant human S100A8. Briefly, GDF9 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to S100A8-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GDF9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of GDF9 and S100A8 was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA427Hu01 | 生长分化因子9(GDF9)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA427Hu01 | 生长分化因子9(GDF9)活性蛋白 | Cell culture; Activity Assays. |
| PAA427Hu01 | 生长分化因子9(GDF9)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA427Hu22 | 生长分化因子9(GDF9)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA427Hu | 生长分化因子9(GDF9)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA427Hu | 生长分化因子9(GDF9)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Journal of Women's medicine | Follicular fluid growth differentiation factor-9 concentration and oocyte developmental competence [Ejwm: 0637JWM] |
| Journal of Ovarian Research | Dehydroepiandrosterone improves follicular fluid bone morphogenetic protein-16 and accumulated embryo score of infertility patients with diminished ovarian reserve undergoing in vitro fertilization: a randomized controlled trial [Pubmed:25330837] |
| Oncotarget | Nucleoside reverse transcriptase inhibitor-induced rat oocyte dysfunction and low fertility mediated by autophagy [Pubmed:29423092] |
| Development | Oocytes and hypoxanthine orchestrate the G2-M switch mechanism in ovarian granulosa cells [Pubmed: 32620578] |
| Reprod Sci | Compromised Cumulus-Oocyte Complex Matrix Organization and Expansion in Women with PCOS [34748173] |