音猬因子(SHH)活性蛋白
Active Hedgehog Homolog, Sonic (SHH)
HHG1; HLP3; HPE3; SMMCI; SHHN; SHH-N
- 编号APB831Hu61
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性状冻干粉
- 纯度> 97%
- 等电点7.1
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1267 ¥ 3168 ¥ 6336 ¥ 19008 ¥ 47520
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产品包装(模拟)
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产品包装(模拟)
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SDS-PAGE图
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. It has been reported that SHH can induce alkaline phosphatase production in C3H10T1/2 mouse embryonic fibroblast cells. To test the bioactivity of SHH, C3H10T1/2 cells were seeded into 24-well plate at a density of 1x105 cells/mL , and allowed to attach overnight before treated with certain concentrations of SHH for 24h and alkaline phosphatase levels in the cell supernatant were determined by ELISA. Alkaline phosphatase levels in the cell supernatant of C3H10T1/2 cells increased significantly after stimulated with SHH have shown in Figure1.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB831Hu01 | 音猬因子(SHH)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB831Hu61 | 音猬因子(SHH)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB831Hu01 | 音猬因子(SHH)活性蛋白 | Cell culture; Activity Assays. |
| APB831Hu61 | 音猬因子(SHH)活性蛋白 | Cell culture; Activity Assays. |
| PAB831Hu01 | 音猬因子(SHH)多克隆抗体 | WB; IHC; ICC; IP. |
| MAB831Hu22 | 音猬因子(SHH)单克隆抗体 | WB; IHC |
| MAB831Hu21 | 音猬因子(SHH)单克隆抗体 | WB,IHC |
| MAB831Hu24 | 音猬因子(SHH)单克隆抗体 | WB; IHC; ICC; IP. |
| RAB831Hu21 | 音猬因子(SHH)重组抗体 | WB; IF; ICC; IHC; IP; FCM. |
| MAB831Hu23 | 音猬因子(SHH)单克隆抗体 | IHC. |
| SEB831Hu | 音猬因子(SHH)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEB831Hu | 音猬因子(SHH)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB831Hu | 音猬因子(SHH)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| United States Patent Application | Compositions and Methods for Diagnosing Lung Cancer [y2016:0077095.html] |
| Annals of the Rheumatic Diseases | Elevated serum levels of sonic hedgehog are associated with fibrotic and vascular manifestations in systemic sclerosis [pubmed:28495672] |