归巢关联细胞黏附分子(HCAM)活性蛋白
Active Homing Associated Cell Adhesion Molecule (HCAM)
CDW44; CD44; CSPG8; ECMR-III; HCELL; IN; LHR; MC56; MDU2; MDU3; MIC4; MUTCH-I; Pgp1; Hematopoietic Cell E- And L-Selectin Ligand; Chondroitin Sulfate Proteoglycan 8
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Homing Associated Cell Adhesion Molecule (HCAM), also known as CD44, is a ubiquitously expressed transmembrane glycoprotein mediating cell responses to the extracellular microenvironment. CD44 is the major surface hyaluronan (HA) receptor, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CD44 and biotinylated hyaluronan (HA). Briefly, biotin-linked HA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CD44-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 1 hour, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human CD44 and biotinylated HA was shown in Figure 1, the EC50 for this effect is 0.08 ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA670Hu01 | 归巢关联细胞黏附分子(HCAM)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA670Hu61 | 归巢关联细胞黏附分子(HCAM)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA670Hu62 | 归巢关联细胞黏附分子(HCAM)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA670Hu01 | 归巢关联细胞黏附分子(HCAM)活性蛋白 | Cell culture; Activity Assays. |
| APA670Hu62 | 归巢关联细胞黏附分子(HCAM)活性蛋白 | Cell culture; Activity Assays. |
| APA670Hu61 | 归巢关联细胞黏附分子(HCAM)活性蛋白 | Cell culture; Activity Assays. |
| CPA670Hu21 | 归巢关联细胞黏附分子(HCAM)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA670Hu01 | 归巢关联细胞黏附分子(HCAM)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA670Hu08 | 归巢关联细胞黏附分子(HCAM)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA670Hu61 | 归巢关联细胞黏附分子(HCAM)多克隆抗体(吲哚菁绿标记) | WB; IHC; ICC; IF. |
| MAA670Hu28 | 归巢关联细胞黏附分子(HCAM)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA670Hu21 | 归巢关联细胞黏附分子(HCAM)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA670Hu22 | 归巢关联细胞黏附分子(HCAM)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA670Hu23 | 归巢关联细胞黏附分子(HCAM)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA670Hu24 | 归巢关联细胞黏附分子(HCAM)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA670Hu27 | 归巢关联细胞黏附分子(HCAM)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA670Hu29 | 归巢关联细胞黏附分子(HCAM)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA670Hu25 | 归巢关联细胞黏附分子(HCAM)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA670Hu26 | 归巢关联细胞黏附分子(HCAM)单克隆抗体 | WB; IHC; ICC; IP. |
| FAA670Hu02 | 抗归巢关联细胞黏附分子(HCAM)单克隆抗体 | FCM |
| FAA670Hu04 | 抗归巢关联细胞黏附分子(HCAM)单克隆抗体 | FCM |
| FAA670Hu06 | 抗归巢关联细胞黏附分子(HCAM)单克隆抗体 | FCM |
| FAA670Hu08 | 抗归巢关联细胞黏附分子(HCAM)单克隆抗体 | FCM |
| SEA670Hu | 归巢关联细胞黏附分子(HCAM)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA670Hu | 归巢关联细胞黏附分子(HCAM)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Cancers | Predictive Value of Soluble PD-1, PD-L1, VEGFA, CD40 Ligand and CD44 for Nivolumab Therapy in Advanced Non-Small Cell Lung Cancer: A Case-Control Study [Pubmed: 32085544] |