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归巢关联细胞黏附分子(HCAM)活性蛋白

Active Homing Associated Cell Adhesion Molecule (HCAM)

CDW44; CD44; CSPG8; ECMR-III; HCELL; IN; LHR; MC56; MDU2; MDU3; MIC4; MUTCH-I; Pgp1; Hematopoietic Cell E- And L-Selectin Ligand; Chondroitin Sulfate Proteoglycan 8

  • 归巢关联细胞黏附分子(HCAM)活性蛋白 产品包装(模拟)
  • 归巢关联细胞黏附分子(HCAM)活性蛋白 产品包装(模拟)
  • APA670Hu62.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Homing Associated Cell Adhesion Molecule (HCAM), also known as CD44, is a ubiquitously expressed transmembrane glycoprotein mediating cell responses to the extracellular microenvironment. CD44 is the major surface hyaluronan (HA) receptor, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CD44 and biotinylated hyaluronan (HA). Briefly, biotin-linked HA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CD44-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 1 hour, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human CD44 and biotinylated HA was shown in Figure 1, the EC50 for this effect is 0.08 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA670Hu01 归巢关联细胞黏附分子(HCAM)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA670Hu61 归巢关联细胞黏附分子(HCAM)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA670Hu62 归巢关联细胞黏附分子(HCAM)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA670Hu01 归巢关联细胞黏附分子(HCAM)活性蛋白 Cell culture; Activity Assays.
APA670Hu62 归巢关联细胞黏附分子(HCAM)活性蛋白 Cell culture; Activity Assays.
APA670Hu61 归巢关联细胞黏附分子(HCAM)活性蛋白 Cell culture; Activity Assays.
CPA670Hu21 归巢关联细胞黏附分子(HCAM)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA670Hu01 归巢关联细胞黏附分子(HCAM)多克隆抗体 WB; IHC; ICC; IP.
PAA670Hu08 归巢关联细胞黏附分子(HCAM)多克隆抗体 WB; IHC; ICC; IP.
LAA670Hu61 归巢关联细胞黏附分子(HCAM)多克隆抗体(吲哚菁绿标记) WB; IHC; ICC; IF.
MAA670Hu28 归巢关联细胞黏附分子(HCAM)单克隆抗体 WB; IHC; ICC; IP.
MAA670Hu21 归巢关联细胞黏附分子(HCAM)单克隆抗体 WB; IHC; ICC; IP.
MAA670Hu22 归巢关联细胞黏附分子(HCAM)单克隆抗体 WB; IHC; ICC; IP.
MAA670Hu23 归巢关联细胞黏附分子(HCAM)单克隆抗体 WB; IHC; ICC; IP.
MAA670Hu24 归巢关联细胞黏附分子(HCAM)单克隆抗体 WB; IHC; ICC; IP.
MAA670Hu27 归巢关联细胞黏附分子(HCAM)单克隆抗体 WB; IHC; ICC; IP.
MAA670Hu29 归巢关联细胞黏附分子(HCAM)单克隆抗体 WB; IHC; ICC; IP.
MAA670Hu25 归巢关联细胞黏附分子(HCAM)单克隆抗体 WB; IHC; ICC; IP.
MAA670Hu26 归巢关联细胞黏附分子(HCAM)单克隆抗体 WB; IHC; ICC; IP.
FAA670Hu02 抗归巢关联细胞黏附分子(HCAM)单克隆抗体 FCM
FAA670Hu04 抗归巢关联细胞黏附分子(HCAM)单克隆抗体 FCM
FAA670Hu06 抗归巢关联细胞黏附分子(HCAM)单克隆抗体 FCM
FAA670Hu08 抗归巢关联细胞黏附分子(HCAM)单克隆抗体 FCM
SEA670Hu 归巢关联细胞黏附分子(HCAM)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA670Hu 归巢关联细胞黏附分子(HCAM)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Cancers Predictive Value of Soluble PD-1, PD-L1, VEGFA, CD40 Ligand and CD44 for Nivolumab Therapy in Advanced Non-Small Cell Lung Cancer: A Case-Control Study [Pubmed: 32085544]
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