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免疫球蛋白D(IgD)活性蛋白

Active Immunoglobulin D (IgD)

IGHD; Constant Region Of Heavy Chain Of Immunoglobulin Delta

  • 免疫球蛋白D(IgD)活性蛋白 产品包装(模拟)
  • 免疫球蛋白D(IgD)活性蛋白 产品包装(模拟)
  • APB941Hu01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Immunoglobulin D (IgD) is an immunoglobulin similar in structure to other immunoglobulin classes. It is composed of heavy and light polypeptide chains, has a sedimentation coefficient of approximately 7S, and can be fragmented into Fab and Fc fragments. It is present in very low concentrations in human blood, and its function is not fully understood. IgD is believed to play a role in the immune system, possibly in the regulation of immune responses or as a receptor on the surface of B cells. It is one of the five major classes of immunoglobulins, alongside IgA, IgE, IgG, and IgM.Besides,Cluster of Differentiation 79B (CD79B) has been identified as an interactor of IgD, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human IgD and recombinant human CD79B.Briefly, IgD was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CD79B-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IgD pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human IgD and recombinant human CD79B was shown in Figure 1, the EC50 for this effect is 0.044ug/mL.

用法

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
NPB941Hu01 免疫球蛋白D(IgD)天然蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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LMB941Hu 免疫球蛋白D(IgD)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
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