白介素1家族成员9(IL1F9)活性蛋白
Active Interleukin 1 Family, Member 9 (IL1F9)
IL-1F9; IL36g; IL-1H1; IL-1RP2; IL1E; IL1H1; IL1RP2; Interleukin 36, Gamma; Interleukin-1 Homolog 1; Interleukin-1 epsilon; Interleukin 1-Related Protein 2
- 编号APL621Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 90%
- 等电点6.3
- 应用Cell culture; Activity Assays.
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- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 2064 ¥ 5160 ¥ 10320 ¥ 30960 ¥ 77400
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产品包装(模拟)
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产品包装(模拟)
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Gene sequencing
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SDS-PAGE图
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Figure. Western Blot; Sample: Recombinant IL1F9, Human.
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通过ISO 9001、ISO 13485质量体系认证
活性实验
IL36G (Interleukin 36 gamma), also known as IL-1F9 and IL-1H1 is a member of the IL-1 family. The receptor for IL36 gamma is reported to be a combination of IL-1 R6/IL-1 R rp2 and IL-1 R3/IL-1 R AcP. Thus we have conducted a binding ELISA assay to detect the interaction of recombinant human IL36G with recombinant human IL1R1. Briefly, IL36G were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1R1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL1R1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL36G with IL1R1 was shown in Figure 1 and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APL621Hu01 | 白介素1家族成员9(IL1F9)活性蛋白 | Cell culture; Activity Assays. |
| RPL621Hu01 | 白介素1家族成员9(IL1F9)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAL621Hu01 | 白介素1家族成员9(IL1F9)多克隆抗体 | WB; IHC; ICC; IP. |
| LAL621Hu71 | 白介素1家族成员9(IL1F9)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAL621Hu22 | 白介素1家族成员9(IL1F9)单克隆抗体 | WB; IHC; ICC; IP. |
| MAL621Hu21 | 白介素1家族成员9(IL1F9)单克隆抗体 | WB; IHC; ICC; IP. |
| SEL621Hu | 白介素1家族成员9(IL1F9)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCL621Hu | 白介素1家族成员9(IL1F9)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LML621Hu | 白介素1家族成员9(IL1F9)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| PLOS ONE | Interleukin (IL)-1β Is a Strong Inducer of IL-36γ Expression in Human Colonic Myofibroblasts [PubMed: 26562662] |
| Journal of Investigative Dermatology | Increased interleukin‐36γ expression in skin and sera of patients with atopic dermatitis and mycosis fungoides/Sézary syndrome [Pubmed:29314201] |
| Journal of Investigative Dermatology | For Review Only [] |
| international immunopharmacology | Acitretin inhibits IL-17A-induced IL-36 expression in keratinocytes by down-regulating IκBζ [Pubmed: 31863918] |
| J Agric Food Chem | Inhibition of HMGB1/TLR4 Signaling Pathway by Digitoflavone: A Potential Therapeutic Role in Alcohol-Associated Liver Disease [Pubmed:35212223] |
| Br J Pharmacol | Modulation of IL‐36‐based inflammatory feedback loop through hepatocytes‐derived IL‐36R‐P2X7R axis improves steatosis in alcoholic steatohepatitis [Pubmed:35481896] |