白介素10受体β(IL10Rb)活性蛋白
Active Interleukin 10 Receptor Beta (IL10Rb)
CDW210b; CD210-B; IL10R-B; IL10-RB; CRF2-4; CRFB4; IL-10R2; Interleukin-10 receptor subunit 2
- 编号APB638Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 97%
- 等电点5.0
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1272 ¥ 3180 ¥ 6360 ¥ 19080 ¥ 47700
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE; Sample: Active recombinant IL10Rb, Human.
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Figure. Western Blot; Sample: Recombinant IL10Rb, Human.
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通过ISO 9001、ISO 13485质量体系认证
活性实验
IL10Rb (Interleukin-10 receptor subunit beta) belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex, which is a cell surface receptor required for the activation of several cytokines, including IL10. Thus, a binding ELISA assay was conducted to detect the interaction of IL10Rb and IL10. Briefly, recombinant human IL10RB were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL10-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL10Rb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL10Rb and IL10 was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB638Hu01 | 白介素10受体β(IL10Rb)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB638Hu61 | 白介素10受体β(IL10Rb)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB638Hu01 | 白介素10受体β(IL10Rb)活性蛋白 | Cell culture; Activity Assays. |
| PAB638Hu01 | 白介素10受体β(IL10Rb)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB638Hu71 | 白介素10受体β(IL10Rb)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAB638Hu21 | 白介素10受体β(IL10Rb)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB638Hu22 | 白介素10受体β(IL10Rb)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB638Hu | 白介素10受体β(IL10Rb)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB638Hu | 白介素10受体β(IL10Rb)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |