白介素17受体D(IL17RD)活性蛋白
Active Interleukin 17 Receptor D (IL17RD)
SEF; IL17RLM; IL-17RD; IL17R-D; IL17Rhom; Interleukin-17 receptor-like protein
- 编号APD349Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 90%
- 等电点8.9
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 2112 ¥ 5280 ¥ 10560 ¥ 31680 ¥ 79200
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产品包装(模拟)
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产品包装(模拟)
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SDS-PAGE图
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Figure. Western Blot; Sample: Recombinant IL17RD, Human.
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通过ISO 9001、ISO 13485质量体系认证
活性实验
IL17RD (interleukin-17 receptor D) is a membrane protein belonging to the IL17R protein family, acting as a component of the interleukin-17 receptor signaling complex. Knowing that the interaction between this protein and IL-17R does not require the interleukin, we have conducted a binding a binding ELISA assay to detect the interaction of recombinant human IL17RD with both recombinant human IL17RA and IL17. Briefly, IL17RD were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated or IL17-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL17RA pAb and anti-IL17 pAb separately, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL17RD with IL17RA and IL17 was shown in Figure 1 and Figure 2 respectively and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APD349Hu01 | 白介素17受体D(IL17RD)活性蛋白 | Cell culture; Activity Assays. |
| RPD349Hu01 | 白介素17受体D(IL17RD)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD349Hu01 | 白介素17受体D(IL17RD)多克隆抗体 | WB; IHC; ICC; IP. |
| FAD349Hu01 | 抗白介素17受体D(IL17RD)多克隆抗体 | Flow cytometry. |