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白介素25(IL25)活性蛋白

Active Interleukin 25 (IL25)

IL17E; IL17-E; Interleukin-17E

  • 白介素25(IL25)活性蛋白 产品包装(模拟)
  • 白介素25(IL25)活性蛋白 产品包装(模拟)
  • APB694Hu01.jpg SDS-PAGE图
  • 白介素25(IL25)活性蛋白 Figure. Western Blot; Sample: Recombinant IL25, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

IL25 (Interleukin-25), also known as IL17E, is reported to induce NF-κB activation, and stimulate the production of IL-8. Besides, this cytokine shares the sequence similarity with IL17, which is considered as a ligand for IL17RA. Thus, a binding ELISA assay was conducted to detect the association of recombinant human IL25 with IL17RA. Briefly, IL25 were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL25 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL25 with IL17RA was shown in Figure 1 and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
APB694Hu61 白介素25(IL25)活性蛋白 Cell culture; Activity Assays.
APB694Hu01 白介素25(IL25)活性蛋白 Cell culture; Activity Assays.
RPB694Hu01 白介素25(IL25)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPB694Hu61 白介素25(IL25)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB694Hu01 白介素25(IL25)多克隆抗体 WB; IHC; ICC; IP.
MAB694Hu22 白介素25(IL25)单克隆抗体 WB; IHC; ICC; IP.
SEB694Hu 白介素25(IL25)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB694Hu 白介素25(IL25)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMB694Hu 白介素25(IL25)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Am J Clin Exp Immunol. Molecular analysis of interleukin-25 exons 1 and 2 and its serum levels in Iranian patients with multiple sclerosis [Pubmed:Pmc4138132]
Journal of Interferon & Cytokine Research Epithelial Cell-Derived Cytokines Contribute to the Pathophysiology of Eosinophilic Chronic Rhinosinusitis [Pubmed:26540312]
Cellular & Molecular Immunology IL-25 stimulates M2 macrophage polarization and thereby promotes mitochondrial respiratory capacity and lipolysis in adipose tissues against obesity. [pubmed:28194019]
Allergology International The effect of calprotectin on TSLP and IL-25 production from airway epithelial cells [pubmed:27475624]
Clinical Immunology A mechanism of interleukin-25 production from airway epithelial cells induced by Japanese cedar pollen [Pubmed:29402661]
Clinical Immunology Toll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposis [Pubmed:30056130]
cyberleninka КЛИНИКО-ЭПИДЕМИОЛОГИЧЕСКАЯ ХАРАКТЕРИСТИКА И ОСОБЕННОСТИ БАЛЛАНСА ЦИТОКИНОВ ПРИ КЛЕЩЕВОМ ЭНЦЕФАЛИТЕ В … []
OncoTargets and Therapy effect of Th9/il-9 on the growth of gastric cancer in nude mice [Pubmed: 30988627]
Cancer Medicine IL‐25 promotes cisplatin resistance of lung cancer cells by activating NF‐κB signaling pathway to increase of major vault protein [Pubmed: 31044552]
Traditional Medicine Research Qingfei oral liquid downregulates TRPV1 expression to reduce airway inflammation and mucus hypersecretion injury caused by respiratory syncytial virus infection … []
Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota []
Pathogens Elevated Levels of IL-33, IL-17 and IL-25 Indicate the Progression from Chronicity to Hepatocellular Carcinoma in Hepatitis C Virus Patients [Pubmed:35056005]
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