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白血病抑制因子(LIF)活性蛋白

Active Leukemia Inhibitory Factor (LIF)

CDF; D-FACTOR; HILDA; MLPLI; Cholinergic Differentiation Factor; Differentiation-Stimulating Factor; Melanoma-Derived LPL Inhibitor; Emfilermin

  • 白血病抑制因子(LIF)活性蛋白 产品包装(模拟)
  • 白血病抑制因子(LIF)活性蛋白 产品包装(模拟)
  • APA085Mu01.png Figure. SDS-PAGE
  • 白血病抑制因子(LIF)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Leukemia inhibitory factor (LIF), is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. LIF as a cytokine also has another fuction including: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. Besides, Colony Stimulating Factor Receptor, Granulocyte (GCSFR) has been identified as an interactor of LIF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse LIF and recombinant mouse GCSFR. Briefly, LIF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to GCSFR-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-LIF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of LIF and GCSFR was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of LIF with GCSFR.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
APA085Mu61 白血病抑制因子(LIF)活性蛋白 Cell culture; Activity Assays.
APA085Mu01 白血病抑制因子(LIF)活性蛋白 Cell culture; Activity Assays.
RPA085Mu01 白血病抑制因子(LIF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA085Mu01 白血病抑制因子(LIF)多克隆抗体 WB; IHC; ICC; IP.
SEA085Mu 白血病抑制因子(LIF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA085Mu 白血病抑制因子(LIF)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
British Journal of Anaesthesia Leukemia inhibitory factor (LIF) potentiates antinociception activity and inhibits tolerance induction of opioids [pubmed:28077540]
Biological Rhythm Research A study of altered cytokine rhythms associated with successful implantation in cows [10.1080/09291016.2017.1361159]
Integrated analysis of cancer stem cells-associated lncRNA-miRNA-mRNA network for ovarian cancer via microarray and Gene Expression Omnibus database []
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