运动因子相关蛋白1(MRP1)活性蛋白
Active Motility Related Protein (MRP1)
CD9; TSPAN29; 5H9; BA2; BTCC-1; DRAP-27; GIG2; MIC3; P24; Tetraspanin 29; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3
- 编号APB097Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点5.9
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1272 ¥ 3180 ¥ 6360 ¥ 19080 ¥ 47700
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Motility Related Protein (MRP1) is a member of the superfamily of ATP-binding cassette (ABC) transporters. It can enable ATPase-coupled inorganic anion transmembrane transporter activity. Involved in anion transmembrane transport ; renal tubular secretion ; and response to toxic substance. It is an integral component of plasma membrane. Besides, ITGb1 has been identified as an interactor of MRP1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human MRP1 and recombinant human ITGb1. Briefly, biotin-linked MRP1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to ITGb1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of MRP1 and ITGb1 was shown in Figure 1, the EC50 for this effect is 0.049 ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APB097Hu01 | 运动因子相关蛋白1(MRP1)活性蛋白 | Cell culture; Activity Assays. |
| EPB097Hu61 | 运动因子相关蛋白1(MRP1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB097Hu61 | 运动因子相关蛋白1(MRP1)活性蛋白 | Cell culture; Activity Assays. |
| RPB097Hu01 | 运动因子相关蛋白1(MRP1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB097Hu01 | 运动因子相关蛋白1(MRP1)多克隆抗体 | WB; IHC; ICC; IP. |
| FAB097Hu01 | 抗运动因子相关蛋白1(MRP1)多克隆抗体 | Flow cytometry. |
| FAB097Hu41 | 抗运动因子相关蛋白1(MRP1)多克隆抗体(藻红素标记) | Flow cytometry. |
| FAB097Hu51 | 抗运动因子相关蛋白1(MRP1)多克隆抗体(别藻蓝蛋白标记) | Flow cytometry. |
| FAB097Hu81 | 抗运动因子相关蛋白1(MRP1)多克隆抗体(异硫氰酸荧光素标记) | Flow cytometry. |
| MAB097Hu22 | 运动因子相关蛋白1(MRP1)单克隆抗体 | WB; IHC; ICC; IP. |
| RAB097Hu21 | 运动因子相关蛋白1(MRP1)重组抗体 | WB; IF; ICC; IHC; IP; FCM. |
| MAB097Hu21 | 运动因子相关蛋白1(MRP1)单克隆抗体 | WB; IHC; ICC; IP. |
| FAB097Hu02 | 抗运动因子相关蛋白1(MRP1)单克隆抗体 | FCM |
| FAB097Hu04 | 抗运动因子相关蛋白1(MRP1)单克隆抗体 | FCM |
| FAB097Hu06 | 抗运动因子相关蛋白1(MRP1)单克隆抗体 | FCM |
| FAB097Hu08 | 抗运动因子相关蛋白1(MRP1)单克隆抗体 | FCM |
| FAB097Hu42 | 抗运动因子相关蛋白1(MRP1)单克隆抗体(藻红素标记) | Flow cytometry. |
| FAB097Hu52 | 抗运动因子相关蛋白1(MRP1)单克隆抗体(别藻蓝蛋白标记) | Flow cytometry. |
| FAB097Hu82 | 抗运动因子相关蛋白1(MRP1)单克隆抗体(异硫氰酸荧光素标记) | Flow cytometry. |
| SEB097Hu | 运动因子相关蛋白1(MRP1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB097Hu | 运动因子相关蛋白1(MRP1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Nanomedicine | Upstream Hedgehog signaling components are exported in exosomes of cervical cancer cell lines [Pubmed: 30265222] |
| Biology-Basel | Biomechanical Properties of Blood Plasma Extracellular Vesicles Revealed by Atomic Force Microscopy [33374530] |