肽-N4-N-乙酰基-β-D-葡萄糖胺基天门冬酰胺酶F(PNGaseF)活性蛋白
Active Peptide-N4-N-Acetyl-Beta-D-Glucosaminyl Asparagine Amidase F (PNGaseF)
Ngl; PNG; PNGase F; Glycopeptide N-glycosidase; N-glycanase
- 编号APX267Ge01
- 物种Pan-species (General，通用) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点8.0
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1728 ¥ 4320 ¥ 8640 ¥ 25920 ¥ 64800
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
PNGase F (Peptide-N-glycosidase F) is a kind of enzymes for the deglycosylation of glycoproteins. The enzyme releases asparagine-linked oligosaccharides from glycoproteins and glycopeptides by hydrolyzing the amide of the asparagine (Asn) side chain. Thus, the activity of recombinant PNGase F measured by deglycosylating Interferon Gamma (IFNg) under denatured conditions. Prepare 10×denaturing buffer(5% SDS,400mM DTT), dilute IFNg to 1 μg/μl by 1×denaturing buffer,then heat the solution to 100℃for 10 minutes to denature the glycoprotein. Cool to room temperature and microcentrifuge briefly. Dobule dilution of recombinant PNGase F by assay buffer(50mmol/L Tris(pH7.4),1% NP-40), add 10 μl denatured IFNg to 10 μl different concentration of recombinant PNGase F, incubate reaction mixture at 37℃ for 1 hour. Stop the reaction by heating to 100℃ for 5 minutes, assess deglycosylation by SDS-PAGE. In the 10 μl reaction system, the amount of PNGase F needed to remove more than 95% carbohydrates from 10 μg denatured IFNg in 1 hour at 37℃ was defined as a unit. In this procedure, one unit is equal to 2.5ng recombinant PNGase F . The results are shown in Figure 1.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Pan-species (General，通用) | 应用(仅供研究使用，不用于临床诊断！) |
| RPX267Ge01 | 肽-N4-N-乙酰基-β-D-葡萄糖胺基天门冬酰胺酶F(PNGaseF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APX267Ge01 | 肽-N4-N-乙酰基-β-D-葡萄糖胺基天门冬酰胺酶F(PNGaseF)活性蛋白 | Cell culture; Activity Assays. |