尿激酶型纤溶酶原激活因子受体(uPAR)活性蛋白
Active Plasminogen Activator, Urokinase Receptor (uPAR)
CD87; PLAUR; PLAU-R; U-PAR; URKR; Monocyte Activation Antigen Mo3
- 编号APA141Mu01
- 物种Mus musculus (Mouse,小鼠) 相同的名称,不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点6.3
- 应用Cell culture; Activity Assays.
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- 规格 10µg50µg 200µg 1mg 5mg
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活性实验
Plasminogen Activator, Urokinase Receptor (uPAR) is the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. The urokinase receptor (uPAR) was identified as the membrane receptor of the serine protease urokinase (uPA), thereby implicated in the plasminogen activation cascade and regulation of pericellular proteolysis, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse uPAR and recombinant human uPA. Briefly, uPAR was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to uPA-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-uPAR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse uPAR and recombinant human uPA was shown in Figure 1, the EC50 for this effect is 0.069 ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
增值服务
相关产品
编号 | 适用物种:Mus musculus (Mouse,小鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPA141Mu01 | 尿激酶型纤溶酶原激活因子受体(uPAR)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA141Mu01 | 尿激酶型纤溶酶原激活因子受体(uPAR)活性蛋白 | Cell culture; Activity Assays. |
RPA141Mu02 | 尿激酶型纤溶酶原激活因子受体(uPAR)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA141Mu01 | 尿激酶型纤溶酶原激活因子受体(uPAR)多克隆抗体 | WB; IHC; ICC; IP. |
PAA141Mu02 | 尿激酶型纤溶酶原激活因子受体(uPAR)多克隆抗体 | WB; IHC; ICC; IP. |
LAA141Mu71 | 尿激酶型纤溶酶原激活因子受体(uPAR)多克隆抗体(生物素标记) | WB; IHC; ICC. |
LAA141Mu81 | 尿激酶型纤溶酶原激活因子受体(uPAR)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
SEA141Mu | 尿激酶型纤溶酶原激活因子受体(uPAR)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA141Mu | 尿激酶型纤溶酶原激活因子受体(uPAR)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
杂志 | 参考文献 |
International Journal of Cancer | Dietary supplementation with methylseleninic acid, but not selenomethionine, reduces spontaneous metastasis of Lewis lung carcinoma in mice [Wiley: source] |
Biomark Insights | Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism [PubMed: PMC3623607] |
Biomarker insights | Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) [PubMed: PMC3762604] |
Am J Emerg Med | Clinical value of soluble urokinase-type plasminogen activator receptor in the diagnosis, prognosis, and therapeutic guidance of sepsis [PubMed: 26615223] |
Archives of Clinical Infectious Diseases | Evaluation of Diagnostic Value of Soluble Urokinase-Type Plasminogen Activator Receptor in Sepsis [Archcid: 26346] |
The American Journal of Emergency Medicine | Clinical value of soluble urokinase-type plasminogen activator receptor in the diagnosis, prognosis, and therapeutic Cavia (Guinea pig )idance of sepsis [science:S0735675715009559] |
Journal of Clinical and Experimental Hepatology | Evaluation of Plasma Urokinase-Type Plasminogen Activator Receptor (UPAR) in Patients With Chronic Hepatitis B, C and Non-Alcoholic Fatty Liver Disease (NAFLD) … [10.1016:j.jceh.2018.02.001] |
Turkish journal of trauma & emergency surgery | The diagnostic value of serum urokinase-type plasminogen activator receptor in acute appendicitis [Pubmed: 31475329] |
CELL TRANSPLANTATION | Predictive Value of Degranulating Factors of Neutrophils in Massive Cerebral Infarction [33787356] |