太阳集团tyc5997_平台(china)官方网站

蛋白酪氨酸磷酸酶受体C(CD45)活性蛋白

Active Protein Tyrosine Phosphatase Receptor Type C (CD45)

PTPRC; PTPR-C; LCA; B220; GP180; LY5; T200; Leukocyte common antigen

  • 蛋白酪氨酸磷酸酶受体C(CD45)活性蛋白 产品包装(模拟)
  • 蛋白酪氨酸磷酸酶受体C(CD45)活性蛋白 产品包装(模拟)
  • APB030Hu61.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Protein Tyrosine Phosphatase Receptor Type C (CD45) is one of the most abundant leukocyte cell surface glycoproteins and is expressed exclusively upon cells of the hematopoietic system. CD45 functions positively to regulate lymphocyte activation by servesing to dephosphorylate and activate members of the Src-tyrosine kinase family. it has been reported that SEMA4D can associate with the phosphatase CD45 at the surface of T cells and that triggering of CD45 on T cells, using monoclonal antibodies (mAb), induces shedding of SEMA4D. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CD45 and recombinant human SEMA4D. Briefly, biotin-linked CD45 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to SEMA4D-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of CD45 and SEMA4D was shown in Figure 1, the EC50 for this effect is 6.47 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPB030Hu03 蛋白酪氨酸磷酸酶受体C(CD45)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB030Hu01 蛋白酪氨酸磷酸酶受体C(CD45)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB030Hu61 蛋白酪氨酸磷酸酶受体C(CD45)活性蛋白 Cell culture; Activity Assays.
RPB030Hu02 蛋白酪氨酸磷酸酶受体C(CD45)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPB030Hu61 蛋白酪氨酸磷酸酶受体C(CD45)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB030Hu02 蛋白酪氨酸磷酸酶受体C(CD45)多克隆抗体 WB; IHC; ICC; IP.
PAB030Hu01 蛋白酪氨酸磷酸酶受体C(CD45)多克隆抗体 WB; IHC; ICC; IP.
FAB030Hu01 抗蛋白酪氨酸磷酸酶受体C(CD45)多克隆抗体 FCM
LAB030Hu71 蛋白酪氨酸磷酸酶受体C(CD45)多克隆抗体(生物素标记) WB; IHC; ICC.
MAB030Hu23 蛋白酪氨酸磷酸酶受体C(CD45)单克隆抗体 WB; IHC; ICC; IP.
MAB030Hu24 蛋白酪氨酸磷酸酶受体C(CD45)单克隆抗体 WB; IHC; ICC; IP.
RAB030Hu21 蛋白酪氨酸磷酸酶受体C(CD45)重组抗体 WB; IHC; ICC; FCM.
MAB030Hu22 蛋白酪氨酸磷酸酶受体C(CD45)单克隆抗体 WB; IHC; ICC; IP.
MAB030Hu21 蛋白酪氨酸磷酸酶受体C(CD45)单克隆抗体 WB; IHC; ICC; IP.
RAB030Hu22 蛋白酪氨酸磷酸酶受体C(CD45)重组抗体 WB; IF; ICC; IHC; IP; FCM.
RAB030Hu01 蛋白酪氨酸磷酸酶受体C(CD45)重组抗体 WB; IF; ICC; IHC; IP; FCM.
FAB030Hu86 抗蛋白酪氨酸磷酸酶受体C(CD45)单克隆抗体(异硫氰酸荧光素标记) FCM
FAB030Hu34 抗蛋白酪氨酸磷酸酶受体C(CD45)单克隆抗体(别藻蓝蛋白/花青染料cy7标记) FCM

参考文献

杂志 参考文献
Cell Size-dependent segregation controls macrophage phagocytosis of antibody-opsonized targets [Pubmed:29958103]
留言咨询
Baidu
sogou