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S100钙结合蛋白A11(S100A11)活性蛋白

Active S100 Calcium Binding Protein A11 (S100A11)

S100-A11; S100C; MLN70; Protein S100-C; Calgizzarin; Metastatic lymph node gene 70 protein; Protein S100-A11, N-terminally processed

  • S100钙结合蛋白A11(S100A11)活性蛋白 产品包装(模拟)
  • S100钙结合蛋白A11(S100A11)活性蛋白 产品包装(模拟)
  • APA568Ra01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

S100 Calcium Binding Protein A11 (S100A11) is a member of the S100 calcium-binding protein family, it contains two EF-hand calcium-binding motifs and shares 82% amino acid sequence identity with mouse and rat S100A11. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in motility, invasion and tubulin polymerization. Besides, S100 Calcium Binding Protein A10 (S100A10) has been identified as an interactor of S100A11, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat S100A11 and recombinant human S100A10. Briefly, S100A11 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to S100A10-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-S100A11 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant rat S100A11 and recombinant human S100A10 was shown in Figure 1, the EC50 for this effect is 0.19 ug/ml.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
APA568Ra01 S100钙结合蛋白A11(S100A11)活性蛋白 Cell culture; Activity Assays.
RPA568Ra01 S100钙结合蛋白A11(S100A11)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA568Ra01 S100钙结合蛋白A11(S100A11)多克隆抗体 WB; IHC; ICC; IP.
LAA568Ra71 S100钙结合蛋白A11(S100A11)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA568Ra22 S100钙结合蛋白A11(S100A11)单克隆抗体 WB; IHC; ICC; IP.
MAA568Ra21 S100钙结合蛋白A11(S100A11)单克隆抗体 WB; IHC; ICC; IP.
SEA568Ra S100钙结合蛋白A11(S100A11)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA568Ra S100钙结合蛋白A11(S100A11)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Gastroenterology Tu1938-Micronutrients Assessment in Patients with Cirrhosis [10.1016:S0016-5085(18)33544-3]
Gastroenterology Tu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis [10.1016:S0016-5085(18)33544-5]
Journal of Proteomics Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection [Pubmed: 31706945]
scandinavian journal of clinical & laboratory investigation Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study [Pubmed: 31856598]
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