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胞外超氧化物歧化酶(SOD3)活性蛋白

Active Superoxide Dismutase 3, Extracellular (SOD3)

SOD-3; EC-SOD; Extracellular Superoxide Dismutase Cu-Zn

  • 胞外超氧化物歧化酶(SOD3)活性蛋白 产品包装(模拟)
  • 胞外超氧化物歧化酶(SOD3)活性蛋白 产品包装(模拟)
  • APA117Mu61.jpg SDS-PAGE图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Extracellular superoxide dismutase [Cu-Zn] is an enzyme that in humans is encoded by the SOD3 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Acroding to the report, in a weakly alkaline buffer solution (pH=8.2) with N-tris(hydroxymethyl)amino methane-HCL, pyrogallol can occur autoxidation in the air, then SOD can inhibit this reaction. Thus, we use this way to measued the activity of recombinant mouse SOD3. The reaction was performed in adding 5 μl 5 mmol/L pyrogallol to 200μl 50mmol/L Tris-HCl, rapidly mixing at 25 ℃, then read at 325 nm (using 50mmol/L Tris-HCl as blank control) in kinetic mode for 3 minutes using a microplate reader controlling the pyrogallol autoxidation rate at 0.70 OD/min. Different concentrations of recombinant mouse SOD3 were added into 200 μl 50 mmol/L Tris-HCl, incubated for 20min at 25 ℃, then adding 5μl 5mmol/L pyrogallol to each well, rapidly mixing and read at 325 nm in kinetic mode for 3 minutes. Under these conditions, the enzyme amount of 50% inhibition of pyrogallol autooxidation per minute is defined as a unit. The specific activity of recombinant mouse SOD3 is 127.3 U/mg.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
APA117Mu01 胞外超氧化物歧化酶(SOD3)活性蛋白 Cell culture; Activity Assays.
EPA117Mu61 胞外超氧化物歧化酶(SOD3)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA117Mu01 胞外超氧化物歧化酶(SOD3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA117Mu02 胞外超氧化物歧化酶(SOD3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA117Mu61 胞外超氧化物歧化酶(SOD3)活性蛋白 Cell culture; Activity Assays.
PAA117Mu01 胞外超氧化物歧化酶(SOD3)多克隆抗体 WB; IHC; ICC; IP.
PAA117Mu02 胞外超氧化物歧化酶(SOD3)多克隆抗体 WB; IHC; ICC; IP.
FAA117Mu01 抗胞外超氧化物歧化酶(SOD3)多克隆抗体 Flow cytometry.
LAA117Mu81 胞外超氧化物歧化酶(SOD3)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA117Mu71 胞外超氧化物歧化酶(SOD3)多克隆抗体(生物素标记) WB; IHC; ICC.
SEA117Mu 胞外超氧化物歧化酶(SOD3)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA117Mu 胞外超氧化物歧化酶(SOD3)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA117Mu 胞外超氧化物歧化酶(SOD3)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA117Mu01 胞外超氧化物歧化酶(SOD3)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Current Alzheimer Research Comparison of Extracellular and Intracellular Blood Compartments Highlights Redox Alterations in Alzheimer's and Mild Cognitive Impairment Patients. [pubmed:27748187]
Frontiers in Microbiology Burn Serum Increases Staphylococcus aureus Biofilm Formation via Oxidative Stress. [pubmed:28702016]
Nature Communications SOD3 improves the tumor response to chemotherapy by stabilizing endothelial HIF-2α [Pubmed:29422508]
BMC Neuroscience Neuroprotective dobutamine treatment upregulates superoxide dismutase 3, anti-oxidant and survival genes and attenuates genes mediating inflammation [Pubmed:29523072]
Diabetes-Metabolism Research and Reviews Myeloperoxidase, superoxide dismutase‐3, cardiometabolic risk factors, and distal sensorimotor polyneuropathy: The KORA F4/FF4 study [Pubmed:29577557]
Advances in Medical Sciences Selected elements of extracellular matrix of the skin in diabetes and insulin resistance [Pubmed: 31146169]
Clinical Kidney Journal Serum uromodulin is inversely associated with biomarkers of subclinical inflammation in the population-based KORA F4 study []
PLoS One Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study [Pubmed:34990470]
ORIGINAL RESEARCH PATHOPHYSIOLOGY EFFECTS OF HIS-PHE-ARG-TRP-PRO-GLY-PRO PEPTIDE ON FREE-RADICAL OXIDATION PROCESSES IN CONDITIONS OF CHRONIC RESTRAINT … []
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