胸腺素β4(TMSB4X)活性蛋白
Active Thymosin Beta 4 (TMSB4X)
Tb4; FX; PTMB4; TB4X; TMSB4; Thymosin,Beta 4,X-Linked; TB-500; Seraspenide; Hematopoietic system regulatory peptide
- 编号APB609Hu03
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点6.0
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 888 ¥ 2220 ¥ 4440 ¥ 13320 ¥ 33300
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Thymosin beta 4 (T beta 4; also TB4X and Fx) is a 5.0 kDa member of the beta-thymosin family of molecules. It Plays an important role in the organization of the cytoskeleton. It is reported that TMSB4X can combinant with PFN1 to inhibit actin polymerization. A binding ELISA assay was conducted to detect the interaction of recombinant human TMSB4X and recombinant human PFN1. Briefly, TMSB4X were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to PFN1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TMSB4X pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TMSB4X and PFN1 was shown in Figure 1, the EC50 for this effect is 2.98 ng/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB609Hu01 | 胸腺素β4(TMSB4X)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB609Hu61 | 胸腺素β4(TMSB4X)活性蛋白 | Cell culture; Activity Assays. |
| RPB609Hu03 | 胸腺素β4(TMSB4X)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB609Hu01 | 胸腺素β4(TMSB4X)活性蛋白 | Cell culture; Activity Assays. |
| APB609Hu03 | 胸腺素β4(TMSB4X)活性蛋白 | Cell culture; Activity Assays. |
| EPB609Hu61 | 胸腺素β4(TMSB4X)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB609Hu02 | 胸腺素β4(TMSB4X)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPB609Hu11 | 胸腺素β4(TMSB4X)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPB609Hu21 | 胸腺素β4(TMSB4X)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAB609Hu03 | 胸腺素β4(TMSB4X)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB609Hu01 | 胸腺素β4(TMSB4X)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB609Hu08 | 胸腺素β4(TMSB4X)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB609Hu71 | 胸腺素β4(TMSB4X)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAB609Hu21 | 胸腺素β4(TMSB4X)单克隆抗体 | WB; IHC; ICC; IP. |
| CEB609Hu | 胸腺素β4(TMSB4X)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB609Hu | 胸腺素β4(TMSB4X)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Scientific Reports | Potential biomarkers of childhood brain tumor identified by proteomics of cerebrospinal fluid from extraventricular drainage (EVD) [33469081] |