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组织因子(TF)活性蛋白

Active Tissue Factor (TF)

CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III

  • 组织因子(TF)活性蛋白 产品包装(模拟)
  • 组织因子(TF)活性蛋白 产品包装(模拟)
  • APA524Ra01.jpg SDS-PAGE图
  • 组织因子(TF)活性蛋白 Figure. Western Blot; Sample: Recombinant Tissue Factor, Rat.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

TF (Tissue factor) also known for platelet tissue factor, factor III, thromboplastin, or CD142, is a cell surface glyprotein present in subendothelial tissue and leukocytes. TF is necessary for the initiation of thrombin formation from the zymogen prothrombin. It has been proven that EGF can bind with the TF on the hemangioendotheliocytes. Thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TF and recombinant rat EGF. Briefly, TF were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL TF were then transferred to EGF-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TF and EGF was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
RPA524Ra01 组织因子(TF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA524Ra01 组织因子(TF)活性蛋白 Cell culture; Activity Assays.
PAA524Ra01 组织因子(TF)多克隆抗体 WB; IHC; ICC; IP.
LAA524Ra81 组织因子(TF)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA524Ra71 组织因子(TF)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA524Ra21 组织因子(TF)单克隆抗体 WB; IHC; ICC; IP.
SEA524Ra 组织因子(TF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA524Ra 组织因子(TF)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Peptides Calcitonin gene-related peptide-mediated antihypertensive and anti-platelet effects by rutaecarpine in spontaneously hypertensive rats [PubMed: 18625276]
Canadian Journal of Physiology and Pharmacology Increased plasma level of asymmetric dimethylarginine in hypertensive rats facilitates platelet aggregation: role of plasma tissue factor [Nrcresearchpress: y10-115]
International immunopharmacology Ruscogenin attenuates monocrotaline-induced pulmonary hypertension in rats [Pubmed: 23538027]
BioMed research international Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations [Pubmed: 24199192]
The Journal of Biological Chemistry Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during Pregnancy [Pubmed:25505239]
Hippokratia Thrombosis in Hemodialysis Patients; Their Association with Tissue Factor and Tissue Factor Pathway Inhibitor [PubMed: 20011089]
shock Noble-Collip Drum Trauma Induces Disseminated Intravascular Coagulation But Not Acute Coagulopathy of Trauma-Shock [PubMed: 25423126]
Perfusion Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testing [PubMed: 26243277]
Molecular Nutrition & Food Research Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors [PubMed: 25546502]
Food Funct The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice [Pubmed:26757699]
Blood Cells, Inflammation and Infection Nebulized Heparin Attenuates Pulmonary Coagulopathy and Inflammation through Alveolar Macrophages in a Rat Model of Acute Lung Injury [pubmed:29202212]
Thrombosis andHaemostasis Nebulized anti-coagulants as a therapy for acute lung injury and acute respiratory distress syndrome [Pubmed: 29202212]
Journal of Thrombosis and Haemostasis Effects of nebulized antithrombin and heparin on inflammatory and coagulation alterations in an acute lung injury model in rats [Pubmed: 31755229]
Mediators Inflamm Amelioration of Coagulation Disorders and Inflammation by Hydrogen-Rich Solution Reduces Intestinal Ischemia/Reperfusion Injury in Rats through NF-κB … [Pubmed: 32587471]
International Journal of Clinical Practice Effect of Glucocorticoid Administration in Intravenous Pulses on Selected Parameters of the Coagulation System [Pubmed:35685509]
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