组织因子通道抑制因子2(TFPI2)活性蛋白
Active Tissue Factor Pathway Inhibitor 2 (TFPI2)
PP5; Placental protein 5
- 编号APB940Mu01
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 95%
- 等电点8.9
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1944 ¥ 4860 ¥ 9720 ¥ 29160 ¥ 72900
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产品包装(模拟)
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产品包装(模拟)
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Figure. Gene Sequencing (Extract)
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Figure. SDS-PAGE
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Figure. Western Blot
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Tissue Factor Pathway Inhibitor 2 (TFPI2) takes part in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. TFPI2 doesn’t have any influence on thrombin. TFPI2 also can inhibit MMP activity, which can hydrolyze gelatin under certain conditions. Thus, the activity of TFPI2 can be measured by inhibit MMP-2 hydrolyze gelatin. Gelatin zymography is mainly used for the detection of the gelatinases, 2μg/mL was denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate–polyacrylamide gel (SDS–PAGE; 8% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubate with various concentrations of recombinant mouse TFPI2, then staining with coomassie brilliant blue G250, active MMP-2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel; if the activity of MMP-2 inhibit by TFPI2, there was none white binds on the gel.The result was shown in figure 1.
As the figure 1 shown, MMP-2 can be inhibited by recombinant mouse TFPI2 at least 2.5μg/mL.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB940Mu01 | 组织因子通道抑制因子2(TFPI2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB940Mu01 | 组织因子通道抑制因子2(TFPI2)活性蛋白 | Cell culture; Activity Assays. |
| PAB940Mu01 | 组织因子通道抑制因子2(TFPI2)多克隆抗体 | WB; IHC; ICC; IP. |
| SEB940Mu | 组织因子通道抑制因子2(TFPI2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB940Mu | 组织因子通道抑制因子2(TFPI2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PGB940Mu01 | 组织因子通道抑制因子2(TFPI2)引物对 | PCR |