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组织型纤溶酶原激活因子(tPA)活性蛋白

Active Tissue Plasminogen Activator (tPA)

PLAT; T-PA; t-plasminogen activator; Alteplase; Reteplase

  • 组织型纤溶酶原激活因子(tPA)活性蛋白 产品包装(模拟)
  • 组织型纤溶酶原激活因子(tPA)活性蛋白 产品包装(模拟)
  • APA525Hu61.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Plasminogen activators are serine proteases that catalyze the activation of plasmin via proteolytic cleavage of its zymogen form plasminogen. Plasmin is an important factor in fibrinolysis, the breakdown of fibrin polymers formed during blood clotting. There are two main plasminogen activators: urokinase (uPA) and tissue plasminogen activator (tPA). Tissue plasminogen activators (tPA) are used to treat medical conditions related to blood clotting including embolic or thrombotic stroke, myocardial infarction, and pulmonary embolism. The activity assay of tPA was measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The reaction was performed in 50 mM Tris, 0.01% Tween-20, pH 8.5 ( Assay Buffer), ainitiated by addition 50 μL of various concentrations of tPA (diluted by Assay Buffer) to 50 µL of 200 uM Substrate. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes. The specific activity of recombinant human tPA is 10-60 pmol/min/µg.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPA525Hu04 组织型纤溶酶原激活因子(tPA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA525Hu05 组织型纤溶酶原激活因子(tPA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA525Hu06 组织型纤溶酶原激活因子(tPA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA525Hu02 组织型纤溶酶原激活因子(tPA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA525Hu62 组织型纤溶酶原激活因子(tPA)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA525Hu01 组织型纤溶酶原激活因子(tPA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA525Hu03 组织型纤溶酶原激活因子(tPA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA525Hu61 组织型纤溶酶原激活因子(tPA)活性蛋白 Cell culture; Activity Assays.
APA525Hu62 组织型纤溶酶原激活因子(tPA)活性蛋白 Cell culture; Activity Assays.
EPA525Hu61 组织型纤溶酶原激活因子(tPA)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA525Hu01 组织型纤溶酶原激活因子(tPA)多克隆抗体 WB; IHC; ICC; IP.
PAA525Hu02 组织型纤溶酶原激活因子(tPA)多克隆抗体 WB; IHC; ICC; IP.
MAA525Hu22 组织型纤溶酶原激活因子(tPA)单克隆抗体 WB; IHC; ICC; IP.
MAA525Hu24 组织型纤溶酶原激活因子(tPA)单克隆抗体 WB; IHC; ICC; IP.
MAA525Hu26 组织型纤溶酶原激活因子(tPA)单克隆抗体 WB; IHC; ICC; IP.
MAA525Hu23 组织型纤溶酶原激活因子(tPA)单克隆抗体 WB; IHC; ICC; IP.
MAA525Hu21 组织型纤溶酶原激活因子(tPA)单克隆抗体 WB; IHC; ICC; IP.
MAA525Hu28 组织型纤溶酶原激活因子(tPA)单克隆抗体 WB; IHC; ICC; IP.
MAA525Hu25 组织型纤溶酶原激活因子(tPA)单克隆抗体 WB; IHC; ICC; IP.
MAA525Hu27 组织型纤溶酶原激活因子(tPA)单克隆抗体 WB; IHC; ICC; IP.
MAA525Hu29 组织型纤溶酶原激活因子(tPA)单克隆抗体 WB; IHC; ICC; IP.
SEA525Hu 组织型纤溶酶原激活因子(tPA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA525Hu 组织型纤溶酶原激活因子(tPA)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Annals of Clinical & Laboratory Science Alteration of Hemostatic Parameters in Patients with Different Levels of Subclinical Hypothyroidism and the Effect of L-thyroxine Treatment [pubmed:28249913]
Cellular Physiology and Biochemistry Defective Platelet Activation and Bleeding Complications upon Cholestasis in Mice [pubmed:28441661]
Journal of allergy and clinical immunology Cytokine and estrogen stimulation of endothelial cells augments activation of the prekallikrein-high molecular weight kininogen complex: Implications for hereditary angioedema. [pubmed:27826093]
European Journal of Neuroscience Urokinase receptor and tissue plasminogen activator as immediate‐early genes in pentylenetetrazole‐induced seizures in the mouse brain [Pubmed: 31587391]
Phytomedicine Sodium aescinate significantly suppress postoperative peritoneal adhesion by inhibiting the RhoA/ROCK signaling pathway [Pubmed: 32120245]
Sci Rep Fibroblast growth factor-21 as a novel metabolic factor for regulating thrombotic homeostasis [Pubmed:35013379]
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