VGF神经生长因子诱导蛋白(VGF)活性蛋白
Active VGF Nerve Growth Factor Inducible (VGF)
NERP1; NERP2; Neuro-Endocrine Specific Protein VGF; Neuroendocrine Regulatory Peptide
- 编号APB166Mu61
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性状冻干粉
- 纯度> 95%
- 等电点4.2
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 2232 ¥ 5580 ¥ 11160 ¥ 33480 ¥ 83700
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产品包装(模拟)
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产品包装(模拟)
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SDS-PAGE图
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Neurosecretory protein VGF is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The protein shares similarities with the secretogranin/chromogranin family, however, its exact function is not known. Besides, Early Growth Response Protein 1 (EGR1) has been identified as an interactor of VGF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse VGF and recombinant mouse EGR1. Briefly, EGR1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to VGF-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti- EGR1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450/630nm immediately. The binding activity of VGF and EGR1 was shown in Figure 1, the ED50 for this effect is 0.515 ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| EPB166Mu61 | VGF神经生长因子诱导蛋白(VGF)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB166Mu02 | VGF神经生长因子诱导蛋白(VGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB166Mu01 | VGF神经生长因子诱导蛋白(VGF)活性蛋白 | Cell culture; Activity Assays. |
| APB166Mu61 | VGF神经生长因子诱导蛋白(VGF)活性蛋白 | Cell culture; Activity Assays. |
| RPB166Mu01 | VGF神经生长因子诱导蛋白(VGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB166Mu01 | VGF神经生长因子诱导蛋白(VGF)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB166Mu02 | VGF神经生长因子诱导蛋白(VGF)多克隆抗体 | WB; IHC; ICC; IP. |
| SEB166Mu | VGF神经生长因子诱导蛋白(VGF)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB166Mu | VGF神经生长因子诱导蛋白(VGF)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Journal of Neuroendocrinology | Proteomic analysis of cerebrospinal fluid from obese women with idiopathic intracranial hypertension: a new approach for identifying new candidates in the pathogenesis of obesity [PubMed: 22296024] |
| J Psychiatr Res | Low VGF is associated with executive dysfunction in patients with major depressive disorder [Pubmed:35738161] |