载脂蛋白E(APOE)重组蛋白
Recombinant Apolipoprotein E (APOE)
Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset
- 编号RPA704Mu01
- 物种Mus musculus (Mouse,小鼠) 相同的名称,不同的物种。
- 来源原核表达
- 宿主E.coli
- 内毒素水平<1.0EU/µg(LAL法测定)
- 亚细胞定位分泌
- 预测分子量48.0kDa
- 实际分子量48kDa(差异分析请参阅说明书)
- 片段与标签Thr14~Lys167 with N-terminal His and GST Tag
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性状冻干粉
- 纯度> 95%
- 等电点5.7
-
应用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,请参见活性蛋白。 - 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1080 ¥ 2700 ¥ 5400 ¥ 16200 ¥ 40500
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
增值服务
相关产品
编号 | 适用物种:Mus musculus (Mouse,小鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPA704Mu02 | 载脂蛋白E(APOE)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA704Mu01 | 载脂蛋白E(APOE)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA704Mu01 | 载脂蛋白E(APOE)多克隆抗体 | WB; IHC; ICC; IP. |
PAA704Mu02 | 载脂蛋白E(APOE)多克隆抗体 | WB; IHC; ICC; IP. |
LAA704Mu81 | 载脂蛋白E(APOE)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
LAA704Mu71 | 载脂蛋白E(APOE)多克隆抗体(生物素标记) | WB; IHC; ICC. |
SEA704Mu | 载脂蛋白E(APOE)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA704Mu | 载脂蛋白E(APOE)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA704Mu01 | 载脂蛋白E(APOE)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Seizure - European Journal of Epilepsy | Cerebrospinal fluid apolipoprotein E concentration decreases after seizure [Seizure: PIIS1059131109002441] |
Biochimica et Biophysica Acta | Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties [PubMed: 23328279 ] |
Medical Oncology | Expression and clinical significance of apolipoprotein E in pancreatic ductal adenocarcinoma [Pubmed: 23609192] |
Diabetes. | Adipocyte Atp-Binding Cassette G1 Promotes Triglyceride Storage, Fat Mass Growth And Human Obesity [Pubmed:25249572] |
renal failure | Frequencies of apolipoprotein E alleles in depressed patients undergoing hemodialysis – a case-control study [PubMed: 25707516] |
Biochim Biophys Acta | Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism [PubMed: 26515253] |
Diabetes | Adipocyte ATP-binding cassette G1 promotes triglyceride storage, fat mass growth, and human obesity [PubMed: 25249572] |
Scientific Reports | Adaptive immunity against gut microbiota enhances apoE-mediated immune regulation andreduces atherosclerosis and western-diet-related inflammation. [pubmed:27383250] |
Biochemical Pharmacology | Differential effects of apoE and apoJ mimetic peptides on the action of an anti-Aβ scFv in 3xTg-AD mice [Pubmed:30026023] |
Oncotarget | Deletion of plasma Phospholipid Transfer Protein (PLTP) increases microglial phagocytosis and reduces cerebral amyloid-β deposition in the J20 mouse … [Pubmed:29731975] |
Proteomics Clinical Applications | Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancy [Pubmed: 31162828] |
Nature Communications | Hepatic HuR modulates lipid homeostasis in response to high-fat diet [Pubmed: 32546794] |
VETERINARY PATHOLOGY | Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and Cats [Pubmed: 32880234] |
Journal of Clinical Medicine | The Significance of Apolipoprotein E Measurement in the Screening of Fetal Down Syndrome [33321701] |
Cancer Cell Int | Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer [33602229] |
Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313] |
Talanta | Iridium nanoclusters as high sensitive-tunable elemental labels for immunoassays: Determination of IgE and APOE in aqueous humor by inductively coupled … [Pubmed:35378356] |
Analytica Chimica Acta | Gold nanoclusters as elemental label for the sequential quantification of apolipoprotein E and metallothionein 2A in individual human cells of the retinal … [Pubmed:35361429] |
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