太阳集团tyc5997_平台(china)官方网站

饥饿素(GHRL)检测试剂盒(化学发光免疫分析法)

CLIA Kit for Ghrelin (GHRL)

MTLRP; Growth Hormone-Releasing Peptide

  • 饥饿素(GHRL)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 饥饿素(GHRL)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 饥饿素(GHRL)检测试剂盒(化学发光免疫分析法) 实验结果图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测饥饿素(GHRL),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的饥饿素(GHRL)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 96-105 101
EDTA plasma(n=5) 79-93 89
heparin plasma(n=5) 80-97 93

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的饥饿素(GHRL),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中饥饿素(GHRL)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 80-105% 99-105% 86-94% 90-102%
EDTA plasma(n=5) 94-103% 98-105% 79-96% 78-98%
heparin plasma(n=5) 79-92% 81-90% 97-105% 80-95%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加底物100µL,37°C孵育10分钟;
7. 读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将饥饿素(GHRL)单克隆抗体包被微孔板,制成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的饥饿素(GHRL)呈负相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。

相关产品

编号 适用物种:Chicken (Gallus,鸡) 应用(仅供研究使用,不用于临床诊断!)
CEA991Ga 饥饿素(GHRL)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA991Ga 饥饿素(GHRL)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA991Ga 饥饿素(GHRL)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Archives of Oral Biology Expression of ghrelin in human salivary glands and its levels in saliva and serum in Chinese obese children and adolescents [PubMed: 21075359]
Department of Medical Biochemistry and Clinical Biochemistry Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cows [ScienceDirect: S0196978113000533]
Journal of Physiology and Pharmacology Postnatal administration of 2-oxoglutaric acid improves articular and growth plate cartilages and bone tissue morphology in pigs prenatally treated with dexamethasone [PubMed: 23211309]
Gene The modifying effects of fish oil on fasting ghrelin mRNA expression in weaned rats [PubMed: 22842192]
Hormone Research in Paediatrics Changes in Ghrelin and Obestatin Levels before and after a Meal in Children with Simple Obesity and Anorexia [Pubmed: 23774845]
Surgical Innovation Gastric supply manipulation to modulate ghrelin production and enhance vascularization to the cardia: proof of the concept in a porcine model [Pubmed:25294792]
Obesity Surgery Embolization of Arterial Gastric Supply in Obesity (EMBARGO): an Endovascular Approach in the Management of Morbid Obesity. Proof of the Concept in the Porcine Model [Pubmed:25511751]
Obes Surg Effect of Sleeve Gastrectomy Plus Side-to-Side Jejunoileal Anastomosis for Type 2 Diabetes Control in an Obese Rat Model [PubMed: 26202420]
Surg Innov Gastric Supply Manipulation to Modulate Ghrelin Production and Enhance Vascularization to the Cardia Proof of the Concept in a Porcine Model [PubMed: 25294792]
Nutrients.  Elevation of Fasting Ghrelin in Healthy Human Subjects Consuming a High-Salt Diet: A Novel Mechanism of Obesity? [Pubmed:27240398]
World Journal of Gastroenterology Effects of sleeve gastrectomy with jejuno-jejunal or jejuno-ileal loop onglycolipid metabolism in diabetic rats. [pubmed:27621579]
Ginekologia Polska Increased serum ghrelin in preeclampsia: is ghrelin a friend or a foe? [pubmed:27321099]
Gynecological Endocrinology The association between serum ghrelin levels and large-for-gestational-age fetuses in patients with gestational diabetes mellitus [pubmed:27791436]
Acta Diabetologica Effect of lifestyle improvement program on the biomarkersof adiposity, inflammation and gut hormones in overweight/obese Asian Indians with prediabetes [pubmed:28620678]
Scandinavian Journal of Clinical and Laboratory Investigation Role of serotonin hormone in weight regain after sleeve gastrectomy [pubmed:29228802]
Slovenian Veterinary Research EFFECT OF Garcinia cambogia EXTRACT ON SERUM LEPTIN, GHRELIN, ADIPONECTIN AND INSULIN LEVELS AND BODY WEIGHTS IN RATS FED WITH HIGH … [Slovetres:Source]
The American Journal of the Medical Sciences The Relations Among Serum Ghrelin, Motilin and Gastric Emptying and Autonomic Function in Autoimmune Gastritis [Pubmed:29753372]
American Journal of Perinatology The Role of Umbilical Cord Blood Concentration of IGF-I, IGF-II, Leptin, Adiponectin, Ghrelin, Resistin, and Visfatin in Fetal Growth [Pubmed: 30282106]
Stem Cells and Development The reparative effects of human adipose-derived mesenchymal stem cells in the chemotherapy damaged thymus [Pubmed: 30511904]
The Journal of Nutrition Grape Seed Proanthocyanidin Affects Lipid Metabolism via Changing Gut Microflora and Enhancing Propionate Production in Weaned Pigs [Pubmed: 31175811]
Arch Physiol Biochem Associations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise training [Pubmed: 31290696]
Mol Med Rep Ghrelin system is involved in improvements in glucose metabolism mediated by hyperbaric oxygen treatment in a streptozotocin‑induced type 1 diabetes mouse … [Pubmed: 32901885]
endocrine Omega-3 offers better hypothalamus protection by decreasing POMC expression and elevating ghrelin hormone: a prospective trial to overcome methotrexate … [Pubmed: 32424681]
plos one Serum leptin level is associated with phase angle in CKD5 patients not undergoing dialysis [Pubmed: 30089154]
Physiological and Histopathological Effects of Electroporation Pulse on Stomach of Rats []
International Journal of Livestock Research Effect of Nutritional Supplementation on Treatment of Anoestrus Swamp Buffalo Cows and Heifers []
iraqi journal of science Relationship Between Orexin-A and Insulin Resistance in Patients with Type 2 Diabetes Mellitus []
Indian Journal of Forensic Medicine & Toxicology Altered Serum Marker of Adipokines Profile in Breast Cancer Women []
Br J Nutr The Association of Pre-Pregnancy BMI On Leptin, Ghrelin, Adiponectin, and IGF-1 In Breast Milk, A Case Control Study [34348809]
Nutrients Nutritional Status, Selected Nutrients Intake and Their Relationship with the Concentration of Ghrelin and Adiponectin in Patients with Diabetic Nephropathy [34959967]
scientific reports Effect of Sleeve Gastrectomy, Roux-En-Y Gastric Bypass, and Ileal Transposition on Myocardial Ischaemia–Reperfusion Injury in Non-Obese Non-Diabetic Rats [34903800]
npj Biofilms and Microbiomes The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammal [Pubmed:35379849]
Food Hydrocolloids Creating texturally tuneable, low calorie and palatable noodle-like food assemblies via microfluidics []
留言咨询
Baidu
sogou