白介素4(IL4)检测试剂盒(化学发光免疫分析法)
CLIA Kit for Interleukin 4 (IL4)
BSF1; BCGF1 ;B Cell Stimulatory Factor 1; Lymphocyte stimulatory factor 1; B Cell Growth Factor; Binetrakin; Pitrakinra
- 编号SCA077Ra
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 实验方法双抗夹心
- 反应时长2h, 40min
- 检测范围1.37-1,000pg/mL
- 灵敏度最小可检测剂量小于等于0.49pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3830 ¥ 5472 ¥ 24624 ¥ 46512 ¥ 383040
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
本试剂盒用于检测白介素4(IL4),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的白介素4(IL4)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 78-91 | 81 |
EDTA plasma(n=5) | 91-101 | 94 |
heparin plasma(n=5) | 92-99 | 96 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
线性
在定值血清及血浆样本内加入适量的白介素4(IL4),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中白介素4(IL4)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 87-95% | 92-99% | 92-101% | 84-99% |
EDTA plasma(n=5) | 82-101% | 80-98% | 88-95% | 81-101% |
heparin plasma(n=5) | 79-90% | 88-103% | 92-99% | 91-98% |
稳定性
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分钟;
8. 读数。
实验原理
将白介素4(IL4)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的白介素4(IL4)与连接于固相载体上的抗体结合,然后加入生物素化的白介素4(IL4)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的白介素4(IL4)呈正相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。
增值服务
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
APA077Ra01 | 白介素4(IL4)活性蛋白 | Cell culture; Activity Assays. |
EPA077Ra61 | 白介素4(IL4)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA077Ra01 | 白介素4(IL4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA077Ra61 | 白介素4(IL4)活性蛋白 | Cell culture; Activity Assays. |
PAA077Ra06 | 白介素4(IL4)多克隆抗体 | WB; IHC; ICC; IP. |
PAA077Ra01 | 白介素4(IL4)多克隆抗体 | WB; IHC; ICC; IP. |
LAA077Ra71 | 白介素4(IL4)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAA077Ra22 | 白介素4(IL4)单克隆抗体 | WB; IHC; ICC; IP. |
MAA077Ra21 | 白介素4(IL4)单克隆抗体 | WB; IHC; ICC; IP. |
MAA077Ra26 | 白介素4(IL4)单克隆抗体 | WB; IHC; ICC; IP. |
LAA077Ra72 | 白介素4(IL4)单克隆抗体(生物素标记) | WB; IHC; ICC. |
SEA077Ra | 白介素4(IL4)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA077Ra | 白介素4(IL4)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA077Ra | 白介素4(IL4)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA077Ra | 白介素4(IL4)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
PSA077Ra01 | 白介素4(IL4)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA077Ra01 | 白介素4(IL4)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
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Biomed Pharmacother | Allicin ameliorates renal ischemia/reperfusion injury via inhibition of oxidative stress and inflammation in rats [34426252] |
J Periodontol | Cytokine profile in serum and gingival crevicular fluid of children with inflammatory bowel disease: A case‐control study [34730850] |
Transl Psychiatry | Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive … [34802039] |
Inflamm Res | Dexmedetomidine exerts cerebral protective effects against cerebral ischemic injury by promoting the polarization of M2 microglia via the Nrf2/HO-1/NLRP3 pathway [34767031] |
Animal Nutrition | Optimizing the growth and immune system of dairy calves by subdividing the pre-weaning period and providing different milk volumes for each stage [34786502] |
J Inflamm Res | Clenbuterol, a Selective β2-Adrenergic Receptor Agonist, Inhibits or Limits Post-Stroke Pneumonia, but Increases Infarct Volume in MCAO Mice [Pubmed:35058704] |
Mol Cell Biochem | The microRNA-1278/SHP-1/STAT3 pathway is involved in airway smooth muscle cell proliferation in a model of severe asthma both intracellularly and extracellularly [Pubmed:35157180] |
Toxicology | Protection from Benzene-induced Immune Dysfunction in Mice [Pubmed:35090963] |
Biomed Pharmacother | Jiangu granule ameliorated OVX rats bone loss by modulating gut microbiota-SCFAs-Treg/Th17 axis [Pubmed:35453007] |
Journal of Biomaterials Applications | Photobiomodulation associated with lipid nanoparticles and hyaluronic acid accelerate the healing of excisional wounds [Pubmed:35705485] |
Viruses | Synergistic Pathogenicity by Coinfection and Sequential Infection with NADC30-like PRRSV and PCV2 in Post-Weaned Pigs [Pubmed:35215787] |
researchsquare | Dexmedetomidine Attenuates Hyperalgesia Induced By Brachial Plexus Root Avulsion By Restoring The GLT-1 Function Via PKA Signaling [] |
Mljekarstvo | Th1/Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows [] |